<p>1. Physical Methods for Gene Transfer<br>Mohammad Alsaggar and Dexi Liu<br>2. Non-Viral Gene Delivery Systems by the Combination of Bubble Liposomes and Ultrasound<br>Daiki Omata, Yoichi Negishi, Ryo Suzuki, Yusuke Oda, Yoko Endo-Takahashi and Kazuo Maruyama<br>3. Electroporation Mediated Gene Delivery<br>Jennifer L. Young and David A. Dean<br>4. Hydrodynamic Delivery<br>Takeshi Suda and Dexi Liu<br>5. Sustained Expression From DNA Vectors<br>Suet Ping Wong, Orestis Argyros and Richard P. Harbottle<br>6. Non-coding Oligonucleotides: The Belle of the Ball in Gene Therapy<br>Ka-To Shum and John J. Rossi<br>7. Self-Amplifying Mrna Vaccines<br>Luis A. Brito, Sushma Kommareddy, Domenico Maione, Yasushi Uematsu, Cinzia Giovani, Gillis R. Otten, Dong Yu, Christian W. Mandl, Peter W. Mason, Philip R. Dormitzer , Jeffrey B. Ulmer and Andrew J. Geall<br>8. Gene Electrotransfer Clinical Trials<br>Richard Heller and Loree C. Heller<br></p>