Membrane Proteins – Engineering, Purification and Crystallization

Section I. Membrane Protein Engineering, Solubilization and Purification<br>1. Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes<br>Simon Trowitzsch  & Robert Tampé <br>2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs<br>Elizabeth Mathew and Mark E Dumont<br>3. Membrane preparation and solubilization<br>Ankita Roy <br>4. Amphipathic agents for membrane protein study<br>Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae <br>5. Quantification of detergent using colorimetric methods in membrane protein crystallography<br>Chelsy Prince , Zongchao Jia<br>6. Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction<br>Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla<br>7. Overexpression, Isolation, Purification and Crystallization of NhaA<br>Etana Padan and Manish Dwivedi<br>8. Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coli<br>Stefan Köster, Katharina van Pee, Özkan Yildiz <br>9. Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium  Symporters<br>Satinder K. Singh and Aritra Pal<br>Section II. Generation and Use of Antibody Fragments Against Membrane Proteins<br>10. Generation of recombinant antibody fragments for membrane protein crystallization<br>Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte<br>11. Phage Display Selections for Affinity Reagents to Membrane Proteins in Nanodiscs<br>Pawel K. Dominik, and Anthony A. Kossiakoff<br>12. Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexes<br>Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman<br>Section III. Biophysical Studies of  Membrane Proteins<br>13. Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass Spectrometry<br>Sheng Li, Su Youn Lee and Ka Young Chung<br>14. EPR Studies of Gating Mechanisms in Ion Channels<br>Sudha Chakrapani<br>15. Magic-Angle-Spinning Solid-State NMR of Membrane Proteins<br>Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus<br>16. Solution NMR Structure Determination of Polytopic α-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraints<br>Linda Columbus and Brett Kroncke<br>Section IV. Crystallization of Membrane Proteins<br>17. Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron Crystallography<br>Yusuf M. Uddin and Ingeborg Schmidt-Krey<br>18. Crystallization of Membrane Proteins by Vapor Diffusion<br>Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu<br>19. An empirical approach to bicelle crystallization<br>Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham<br>20. Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteins<br>Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov<br>21. Crystallization of Proteins from Crude Bovine Rod Outer Segments<br>Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski<br>22. Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron Laser<br>Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme<br>Section V. Computational Approaches to Understand Membrane Proteins<br>23. Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity Filters<br>Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan<br>24. Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the ‘Mix-and-Match’ Method<br>M. Gregor Madej<br>25. Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing Approaches<br>Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande