Membrane Proteins – Production and Functional Characterization

Section I: Recombinant Expression of Membrane Proteins<br>1. Engineering Escherichia Coli for Functional Expression of Membrane Proteins <br>Franz Y Ho and Bert Poolman<br>2. ACEMBLing a Multiprotein Transmembrane Complex:  The Functional SecYEG-SecDFYajC-YidC  Holotranslocon Protein Secretase/Insertase<br>Joanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel and Imre Berger<br>3.  Expression and Purification of OsVDAC4<br>Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy and M. K. Mathew<br>4. Membrane Protein Expression in Lactococcus Lactis<br>Martin S. King, Christoph Boes and Edmund R.S. Kunji<br>5. An Unconventional Anaerobic Membrane Protein Production System Based on Wolinella Succinogenes<br>Michael Lafontaine and C. Roy D. Lancaster<br>6. Membrane Protein Expression and Analysis in Yeast<br>Katrien Claes, Mouna Guerfal and Nico Callewaert<br>7. Heterologous Expression of G Protein Coupled Receptors in Yeast<br>Nicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette Byrne<br>8. Recombinant G protein-Coupled Receptor Expression in Saccharomyces Cerevisiae for Protein Characterization<br>Kory M. Blocker, Zachary T. Britton, Andrea N. Naranjo, Patrick M. McNeely, Carissa L. Young and Anne S. Robinson<br>9. Baculovirus Mediated Expression of GPCRs in Insect Cells<br>Tuulia Saarenpää, Veli-Pekka Jaakola and Adrian Goldman<br>10. Expression of Membrane Proteins in the Eyes of Transgenic Drosophila Melanogaster <br>Yvonne Hackmann, Lisa Joedicke, Valérie Panneels and Irmgard Sinning<br>11. Rapid Method to Express and Purify Human Membrane Protein Using the Xenopus Oocyte System for Functional and Low-Resolution Structural Analysis<br>Benjamin Clémençon, Michael Fine, Philipp Schneider and Matthias A. Hediger<br>12. Expression of G protein-coupled Receptors in Mammalian Cells<br>Raja Chakraborty, Bing Xu, Rajinder P. Bhullar and Prashen Chelikani<br>13. Construction of Stable Mammalian Cell Lines for Inducible Expression of G Protein-Coupled Receptors <br>Chikwado A. Opefi, Dale Tranter, Steven O. Smith and Philip J. Reeves.<br>14. Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI– Cells Using a PiggyBac Inducible System<br>Lydia N. Caro, Zhijie Li, James M. Rini, Aidin R. Balo and Oliver P. Ernst<br>15. Semliki Forest Virus-based Expression of Recombinant GPCRs<br>Kenneth Lundstrom<br>16. Screening for Lipid Requirements of Membrane Proteins by Combining Cell-Free Expression with Nanodiscs<br>Erik Henrich, Volker Dötsch, and Frank Bernhard<br>Section II. Reconstitution and Functional Characterization of Membrane Proteins<br>17. Liposome Reconstitution and Transport Assay for Recombinant Transporters<br>Zachary Lee Johnson and Seok-Yong Lee<br>18. Lipid Reconstitution and Recording of Recombinant Ion Channels<br>Randy Stockbridge and Ming-Feng Tsai<br>19. Reconstitution of Membrane Proteins - a GPCR as an Example<br>Alan D. Goddard, Patricia M. Dijkman, Roslin J. Adamson, Rosana Inácio dos Reis and Anthony Watts<br>20. Ion Channel Reporter for Monitoring the Activity of Engineered GPCRs<br>Christophe J. Moreau,  Katarzyna Niescierowicz , Lydia Caro , Jean Revilloud and  Michel Vivaudou<br>21. Methods for Labeling Skeletal Muscle Ion Channels Site-Specifically With Fluorophores Suitable for FRET-based Structural Analysis<br>Mohana Mahalingam and James D. Fessenden<br>22. Structural and Functional Studies of Nirc From Salmonella Typhimurium<br>Adriana Rycovska-Blume,Wei Lü, Susana Andrade, Klaus Fendler and Oliver Einsle<br>23. Surface Plasmon Resonance Analysis of 7-Transmembrane Receptors <br>Tonia Aristotelous, Andrew L. Hopkins and Iva Navratilova<br>24. Crosslinking Strategies to Study Peptide Ligand-Receptor Interactions <br>Jeffery Becker<br>25. From recombinant expression to crystals – A step-by-step guide to GPCR crystallography<br> Arun K. Shukla, Punita Kumari, Eshan Ghosh and Kumari Nidhi<br>26. Structure-based Biophysical Analysis of the Interaction of Rhodopsin with G Protein and Arrestin<br>Martha E. Sommer, Matthias Elgeti, Peter W. Hildebrand, Michal Szczepek, Klaus Peter Hofmann and Patrick Scheerer