Riboswitches as Targets and Tools

1. Design of Transcription Regulating Riboswitches<br>Sven Findei, Manja Wachsmuth, Mario M¨orl, and PeterF. Stadler<br>2. Ligand-Dependent Exponential Amplification of Self-Replicating RNA Enzymes<br>Charles Olea, Jr. and Gerald F. Joyce<br>3. Design of Modular “Plug-and-Play” Expression Platforms Derived From Natural Riboswitches for Engineering Novel Genetically Encodable RNA Regulatory Devices<br>Jeremiah J. Trausch and Robert Batey<br>4. Integrating and Amplifying Signal from Riboswitch Biosensors<br>Michael S. Goodson, Svetlana V. Harbaugh, Yaroslav G. Chushak and Nancy Kelley-Loughnane<br>5. Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription. <br>Norikazu Ichihashi, Shungo Kobori, Tetsuya Yomo<br>6. Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system<br>Atsushi Ogawa<br>7. Chemo-enzymatic Synthesis of Selectively 13C/15N-labeled RNA for NMR Structural and Dynamics Studies<br>Luigi J. Alvarado, Andrew P. Longhini, Regan LeBlanc, Bin Chen, Christoph Kreutz and T. Kwaku Dayie<br>8. In Vitro Analysis of Riboswitch-Spinach Aptamer Fusions as Metabolite-Sensing Fluorescent Biosensors<br>Colleen A. Kellenberger and Ming C. Hammond<br>9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases.<br>Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk<br>10. Adapting CRISPR/Cas9 for Functional Genomics Screens<br>Abba Malina , Alexandra Katigbak , Regina Cencic , Rayelle Itoua Maïga , Francis Robert, Hisashi Miura and Jerry Pelletier<br>11. The Icrispr Platform for Rapid Genome Editing in Human Pluripotent Stem Cells<br>Zengrong Zhu, Federico González and Danwei Huangfu<br>12. Using Riboswitches to Regulate Gene Expression and Define Gene Function in Mycobacteria<br>Erik Van Vlack and Jessica C. Seeliger<br>13. Controlling Expression of Genes in the Unicellular Alga Chlamydomonas Reinhardtii With a Vitamin-Repressible Riboswitch<br>Silvia Ramundo and Jean-David Rochaix<br>14. Conditional Control of Gene Expression by Synthetic Riboswitches in Streptomyces Coelicolor<br>Martin M. Rudolph, Michael-Paul Vockenhuber und Beatrix Suess<br>15. Engineering of Ribozyme-Based Aminoglycoside Switches of Gene Expression by in Vivo Genetic Selection in Saccharomyces Cerevisiae<br>Benedikt Klauser, Charlotte Rehm and  Jörg S. Hartig<br>16. Kinetic Folding Design of Aptazyme-Regulated Expression Devices (aREDs) as Riboswitches for Metabolic Engineering<br>David Sparkman-Yager, Rodrigo A Correa-Rojas and James M Carothers<br>17. Riboselector: Riboswitch-Based Synthetic Selection Device to Expedite Evolution of Metabolite-Producing Microorganisms<br>Sungho Jang, Jina Yang, Sang Woo Seo and Gyoo Yeol Jung<br>18. Fluorescence Assays for Monitoring RNA-Ligand Interactions and Riboswitch-Targeted Drug Discovery Screening<br>J. Liu, C. Zeng, S. Zhou, J.A. Means and  J.V. Hines<br>19. Monitoring Ribosomal Frameshifting as a Platform to Screen Anti-Riboswitch Drug Candidates<br>Chien-Hung Yu and René C. L. Olsthoorn