Reconstituting the Cytoskeleton

<p>Polymer dynamics <br>1.  Actin filament dynamics using microfluidics<br>2.  Bacterial actin-like proteins: purification and characterization of self-assembly properties<br>3.  Quantitative analysis of microtubule self-assembly kinetics and tip structure</p> <p>Polymer nucleation and regulation<br>4.  Biochemical reconstitution of the WAVE regulatory complex<br>5.  Rotational movement of formins evaluated by using single-molecule fluorescence polarization<br>6.  Single molecule studies of actin assembly and disassembly factors<br>7.  Assaying microtubule nucleation by the γ-tubulin ring complex<br>8.  Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins.<br>9.  Generation of differentially modified microtubules using in vitro enzymatic approaches</p> <p>Molecular motor ensembles on natural and engineered cargoes <br>10.  Engineering defined motor ensembles with DNA origami<br>11.  Construction and analyses of elastically-coupled multiple motor systems<br>12.  Reconstitution of cortical dynein function             <br>13.  Reconstitution of microtubule-dependent organelle transport<br>14.  Reconstituting the motility of isolated intracellular cargoes </p> <p>Building higher order networks and interactions<br>15.  Reconstitution of contractile actomyosin arrays<br>16.  Directed actin assembly and motility<br>17.  In vitro reconstitution of dynamic microtubules interacting with actin filament networks<br>18.  Measuring kinetochore-microtubule interaction in vitro<br>19. Micropattern-guided assembly of overlapping pairs of dynamic microtubules  </p> <p>Cell extract systems<br>20.  WAVE regulatory complex activation <br>21.  Dissecting principles governing actin assembly using yeast extracts<br>23.  Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization <br>24.  Spindle movements on immobilized chromatin micropatterns</p>