<p>1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila</p> <p>Drashti Dave, Dorota Wloga, and Jacek Gaertig</p> <p>2. Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes</p> <p>Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill</p> <p>3. Tools for Analyzing Intraflagellar Transport in Trypanosomes</p> <p>Daria Julkowska and Philippe Bastin</p> <p>4. Schmidtea mediterranea: A Model System for Analysis of Motile Cilia</p> <p>Panteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King</p> <p>5. Targeted Gene Silencing by RNA Interference in Chlamydomonas</p> <p>Eun-Jeong Kim and Heriberto Cerutti</p> <p>6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas</p> <p>Dennis Diener</p> <p>7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutants</p> <p>in Chlamydomonas reinhardtii</p> <p>Carlo Iomini, Jacob E. Till, and Susan K. Dutcher</p> <p>8. Recording and Analyzing IFT in Chlamydomonas Flagella</p> <p>William Dentler, Kristyn VanderWaal, and Mary E Porter</p> <p>9. Total Internal Reflection Fluorescence (TIRF) Microscopy</p> <p>of Chlamydomonas Flagella</p> <p>Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and</p> <p>Wallace F. Marshall</p> <p>10. Purification of IFT Particle Proteins and Preparation of Recombinant</p> <p>Proteins for Structural and Functional Analysis</p> <p>Robert H. Behal, Ewelina Betleja, and Douglas G. Cole</p> <p>11. Studying Cilia in Zebrafish</p> <p>Iain Drummond</p> <p>12. Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor</p> <p>Sensory Cilia</p> <p>Christine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse</p> <p>13. Analysis of Intraflagellar Transport in C. elegans Sensory Cilia</p> <p>Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, and Jonathan M. Scholey</p> <p>14. Functional Genomics of Intraflagellar Transport-Associated Proteins in C. elegans</p> <p>Peter N. Inglis, Oliver E. Blacque, and Michel R. Leroux</p> <p>15. Generating Conditional Mutants to Analyze Ciliary Functions:</p> <p>The Use of Cre–Lox Technology to Disrupt Cilia in Specific Organs</p> <p>Amber K. O’Connor, Robert A. Kesterson, and Bradley K. Yoder</p> <p>16. Imaging Intraflagellar Transport in Mammalian Primary Cilia</p> <p>Tatiana Y. Besschetnova, Barnali Roy, and Jagesh V. Shah</p> <p>17. Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants</p> <p>Hyuk W. Ko, Aimin Liu, and Jonathan T. Eggenschwiler</p>