Lynne E. Maquat,
Megerditch Kiledjian
Elsevier Science
e druk, 2008
9780123743787
RNA Turnover in Eukaryotes: Nucleases, Pathways and Analysis of mRNA Decay
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2008
ISBN13: 9780123743787
Rubricering
Onderdeel van serie
Methods in Enzymology
Levertijd ongeveer 8 werkdagen
Samenvatting
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.
Specificaties
ISBN13:9780123743787
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
Three-hybrid analysis to study RNA decay<br>S. cerevisiae (i.e, G18 insertions, mutations in nucleases…) <br>Defining degradative activities in Drosophila melanogaster <br>Defining degradative activities in plants <br>RT-PCR product sequencing of mRNA decay intermediates in mammalian cells after decapping and circularization in vitro <br>Tethering Assays<br>Assays of poly(A) tail length (PAT assay, specific oligo-dT anchors…) and polarity of mRNA decay (Invader Assay)<br>In vivo crosslinking-coupled affinity purification of mRNAs for decay analysis (Streptavidin, biotin…)<br>Classification of ribonucleases by computational analyses<br>Analysis of P bodies in Saccharomyces cerevisiae<br>Analysis of stress granules and P bodies in mammalian cells, including neurons PMR1-mediated mRNA decay <br>No-go mRNA in Saccharomyces cerevisiae<br>mRNA decay mediated by decay factor adaptors (SLBP, PUF, Staufen1…)<br>Non-stop decay in Saccharomyces cerevisiae<br>Measuring ARE-mediated mRNA decay in cells<br>Measuring ARE-mediated mRNA decay in animals<br>Methods to study viral-mediated influences on mRNA decay<br>Measuring Saccharomyces cerevisiae mitochondrial mRNA half-life <br>Measuring mRNA decay in Arabidopsis mitochondria<br>Measuring mRNA decay in human mitochondria <br>Measuring mRNA decay in chloroplasts<br>• Nonsense-Mediated mRNA Decay (how do you study NMD, what defines an <br>In Saccharomyces cerevisiae<br>In Drosophila melanogaster <br>In Caenorhabditis elegans <br>In plants<br>In mammals<br>• Analysis of Nuclear RNA Decay<br>Measuring nuclear RNA decay in Saccharomyces cerevisiae (includes pre-mRNA, mRNA, rRNA, TRAMP complex…) <br>Measuring nuclear RNA decay in mammals<br>• tRNA Decay<br>Decay of improperly modified tRNA decay in Sacharomyces cerevisiae<br>• rRNA Decay<br>Decay of nonfunctional rRNA in Sacharomyces cerevisiae<br>• RNAi-Mediated mRNA Decay<br>Defining RNAi targets (e.g., Ago knockdowns, identifying complementary siRNA/miRNA…)<br>Studying miRNAs in unicellular organisms (Chlamydamones reinhardtii) Studying miRNAs in unicellular organisms: RNAi-dependent mRNA turnover in fission yeast: <br>Assays for microRNA-mediated mRNA decay in Caenhorabditis elegans<br>Analyses of microRNA function in zebra fish<br>Targeted deletion of cell-type specific microRNAs in mice for developmental studies