Translation Initiation: Extract Systems and Molecular Genetics

Chapter 1<br>Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation Initiation<br>William C. Merrick and Diane Barth-Baus<br><br>Chapter 2<br>Studying translational control in Drosophila cell-free systems<br>Fátima Gebauer and Matthias W. Hentze<br><br>Chapter 3<br>Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation<br>Daniel R. Gallie<br><br>Chapter 4<br>Viral translation and replication in vitro<br>Yuri V. Svitkin and Nahum Sonenberg<br><br>Chapter 5<br>A practical approach to isolate 48S complexes: Affinity purification and analyses<br>Nicolas Locker & Peter J. Lukavsky<br><br>Chapter 6<br>Yeast phenotypic assays on translational control<br>Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura Asano<br><br>Chapter 7<br>Localization and characterization of protein-protein interaction sites<br>Chingakham Ranjit Singh and Katsura Asano*<br><br>Chapter 8<br>In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking.<br>Leos Valášek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. Nielsen<br><br>Chapter 9<br>Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B<br>Byung-Sik Shin and Thomas E Dever<br><br>Chapter 10<br>The use of fungal in vitro systems for studying translational regulation<br>Cheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs <br><br>Chapter 11<br>Investigating translation initiation using Drosophila molecular genetics<br>Gritta Tettweiler and Paul Lasko<br><br>Chapter 12<br>Analysis of RNA:protein interactions in vivo: Identification of RNA binding partners of nuclear factor 90<br>Andrew M. Parrott, Melissa R. Walsh and Michael B. Mathews<br><br>Chapter 13<br>Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members<br>Robert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary Jagus<br><br>Chapter 14<br>Tethered function assays: An adaptable approach to study RNA regulatory proteins<br>Jeff Coller. Mary Wickens<br><br>Chapter 15<br>Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs<br>Vincent P. Mauro, Stephen A. Chappell, and John Dresios