DNA Repair, Part A

Chapter 1 <br>Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivo <br>Chapter 2 <br>Oxidative DNA glycolases: Recipes from Cloning to Characterization <br>Chapter 3 <br>Purification and Characterization of NEIL1 and NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized bases <br>Chapter 4 <br>Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-links <br>Chapter 5 <br>Isolation and analyses of MutY and MutY Homologs (MYH) <br>Chapter 6 <br>Use of yeast for detection of endogenous abasic legions, their source and their repair <br>Chapter 7 <br>Activities and Mechanism of Pol Beta <br>Chapter 8 <br>Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenases <br>Chapter 9 <br>Purification and Characterization of DNA Photolyases <br>Chapter 10 <br>Genetic and in vitro assays of DNA deamination <br>Chapter 11 <br>The XPC complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repair <br>Chapter 12 <br>Purification and Characterization of E. coli and Human Nucleotide Excision Repair <br>Chapter 13 <br>Assays for transcription elongation by RNA polymerase II using oligo (dC)-tailed <br>template with single DNA damage <br>Chapter 14 <br>In vivo assays for transcription-coupled repair <br>Chapter 15 <br>TFIIh enzymatic activities in transcription and nucleotide excision repair <br>Chapter 16 <br>An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts <br>Chapter 17 <br>Analysis of the excision step in human DNA mismatch repair <br>Chapter 18 <br>Characterisation of the “mismatch repairosome” and its role in the processing of modified nucleosides in vitro <br>Chapter 19 <br>Analysis of DNA Mismatch Repair in Cellular Response to DNA Damage <br>Chapter 20 <br>Characterization of E. coli Translesion Synthesis Polymerases and Their Accessory Factors <br>Chapter 21 <br>Measuring the Fidelity of Translesion DNA Synthesis <br>Chapter 22 <br>DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function <br>Chapter 23 <br>Purification and Characterization of Escherichia coli DNA Polymerase V <br>Chapter 24 <br>Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization <br>Chapter 25 <br>Localisation of Y-family polymerases and the DNA polymerase switch in mammalian cells <br>Chapter 26 <br>Repair of DNA Double Strand Breaks – in vivo Biochemistry <br>Chapter 27 <br>Assays for nonhomologous end-joining in extracts <br>Chapter 28 <br>Purification and assays of Saccharomyces cerevisiae homologous recombination proteins <br>Chapter 29 <br>Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy <br>Chapter 30 <br>Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases <br>Chapter 31 <br>In Vitro Nonhomologous End Joining Assay <br>Chapter 32 <br>The RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexes <br>Chapter 33 <br>Purification and Biochemical Characterization of ATM and MRN