Cytochrome P450, Part C

Section I: Human Genomics and Genetics <br>Mining Databases for Cytochrome P450 Genes.<br>Sequence Alignments, Variabilities, and Vagaries.<br>Human CYP Allele Database: Submission Criteria Procedures and Objectives.<br>Fine-Scale Mapping of CYP Gene Clusters: An Example from Human CYP4 Family.<br>Detection of Single Nucleotide Polymorphisms in CYP2B6 Gene.<br>Genotyping Human Cytochrome P450 1B1 Variants.<br>Genotyping Human CYP2A Variants.<br><br>Section II: Structure and Mechanism <br>Purification and Crystallization of N-Terminally Truncated Forms of Microsomal Cytochrome P450 2C5.<br>Molecular Replacement in P450 Crystal Structure Determinations.<br>Optical Biosensor and Scanning Probe Microscopy Studies of Cytochrome P450 Interactions with Redox Partners and Phospholipid Layers.<br>Cryoradiolysis for the Study of P450 Reaction Intermediates.<br>Analyzing Binding of N-Terminal Truncated, Microsomal Cytochrome P450s to Membranes.<br>Sensitizer-Linked Substrates and Ligands: Ruthenium Probes of Cytochrome P450 Structure and Mechanism.<br>Combining Pharmacophore and Protein Modeling to Predict CYP450 Inhibitors and Substrates.<br>High Pressure: A New Tool to Study P450 Structure and Function.<br><br>Section III: Regulation of Expression <br>Use of in Vitro PXR Assays to Assess CYP3A4 Induction Potential of Drug Candidates.<br>Analysis of CYP mRNA Expression by Branched DNA Technology.<br>Ligand-Induced Coactivator Recruitment to PPAR—Characterized by Fluorescence Resonance Energy Transfer.<br>Fluorescence-Based Ligand Binding Assays for PPARs.<br>Developing Toxicologically Predictive Gene Sets Using cDNA Microarrays and Bayesian Classification.<br>Direct Expression of Fluorescent Protein-Tagged Nuclear Receptor CAR in Mouse Liver.<br>Application of Fluorescent Differential Display and PPAR—Null Mice to Analyze PPAR Target Genes.<br>Histological and Metabolism Analysis of P450 Expression in the Brain.<br>Proteomic Analysis of Rodent Hepatic Responses to Peroxisome Proliferators.<br><br>Section IV: Metabolism <br>Kinetic Analysis for Multiple Substrate Interaction at the Active Site of Cytochrome P450.<br>Design and Application of Fluorometric Assays for Human Cytochrome P450 Inhibition.<br>Automated Quantitative and Qualitative Analysis of Metabolic Stability: A Process for Compound Selection during Drug Discovery.<br>Characterization of Covalent Adducts to Intact Cytochrome P450s by Mass Spectrometry.<br>Mutagenesis Testing Based on Bacterial Expression of Human P450s.<br>Use of Long-Term Cultures of Human Hepatocytes to Study Cytochrome P450 Gene Expression..<br>Polarized Cell Cultures for Integrated Studies of Drug Metabolism and Transport.<br><br>Section V: Invertebrate P450s <br>Use of Methylotropic Yeast Pichia pastoris for Expression of Cytochromes P450.<br>Partial Recoding of P450 and P450 Reductase cDNAs for Improved Expression in Yeast and Plants.<br>Selective Covalent Labeling with Radiolabeled Suicide Substrates for Isolating P450s.<br>Cloning of cDNAs Encoding P450s in Flavonoid/Isoflavonoid Pathway from Elicited Leguminous Cell Cultures.<br>Selected Cell Cultures and Induction Methods for Cloning and Assaying Cytochromes P450s in Alkaloid Pathways.<br>Isolation and Functional Characterization of Cytochrome P450s in Gibberellin Biosynthesis Pathway.