Chapter 1. Förster Resonance Energy transfer –FRET what is it, why do it, and how <br>it’s done. Robert M. Clegg <br><br>Chapter 2.Frequency domain FLIM theory, Instrumentation and data analysis. Peter J. <br>Verveer and Quentin Hanley. <br><br>Chapter 3. Time Domain FLIM; Theory, Instrumentation. Hans C. Gerritsen, A.V. <br>Agronskaia, A.N. Bader, A. Esposito. <br><br>Chapter 4. Multidimensional fluorescence imaging. James James McGinty, <br>Christopher Dunsby, Egidijus Auksorius, Pieter De Beule, Daniel S. Elson, Neil <br>Galletly, Oliver Hoffman, Gordon Kennedy, Peter M. P. Lanigan, Ian Munro, Björn <br>Önfelt, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, M. John Lever, <br>Andrew J. deMello, Gordon S. Stamp, Mark A. A. Neil and Paul M. W. French <br><br>Chapter 5.Visible fluorescent proteins for FRET. Gert-Jan Kremers and Joachim <br>Goedhart. <br><br>Chapter 6. Small Molecule-based FRET probes. Amanda Cobos Correa, Oliver <br>Wichmann and Carsten Schultz. <br><br>Chapter 7. Filter FRET: quantitative imaging of sensitized emission. Kees Jalink and <br>Jacco van Rheenen. <br><br>Chapter 8. Spectral Imaging and its use in the measurement of Förster Resonance <br>Energy Transfer in living cells. Steven S. Vogel, Paul. S. Blank, Srinagesh V. <br>Koushik and Christopher Thaler. <br><br>Chapter 9. Total Internal Reflection Fluorescence Lifetime Imaging Microscopy. <br>Theodorus W.J. Gadella Jr. <br><br>Chapter 10. FRET and FLIM applications in plants. Riyaz A. Bhat. <br><br>Chapter 11. Biomedical FRET-FLIM applications. Phill B. Jones, Brian J. Bacskai, <br>Bradley T. Hyman. <br><br>Chapter 12. Reflections on FRET imaging: formalism, probes and implementation. <br>Elizabeth A. Jares-Erijman and Thomas M. Jovin.