Supplement I: F4. Polymerase chain reaction: gene detection, inverse PCR, and genetic engineering; J. Slightom, et al. G3. Light microscopic applications of immunocytochemistry; K. Vandenbosch, et al. K3. T-DNA tagging in Arabidopsis thaliana: Cloning by gene disruption; B. Schulz, et al. L1. High level gene expression in plants using RNA viruses as transient expression vectors; C.M. Kearney, et al. L2. Geminivirus mediated gene transfer; D. Bisaro, G. Sunter. L3. Caulimovirus mediated gene transfer; I. Jupin, B. Gronenborn. Supplement II: RNA differential display; M.J. Laskowski. Expression analysis of multigene Families by RFLP-coupled domain-directed differential display (RC4D); A. Fischer, et al. Recombinant aequorin methods for measurement of intracellular calcium in plants; H. Knight, et al. GUS activity staining - A powerful tool in plant molecular biology; Jeong Hee Lee, F. Schöffl. In-Cell RT-PCR in a single, detached plant cell; Ho-Hyung Woo. Supplement III: A3. Liposome-mediated transfer of YAC-DNA to tobacco cells; M. Van Wordragen, et al. B7. Agribacterium germ-line transformation: Transformation of Arabidopsis without tissue culture; A.F. Bent, S. Clough. D6. RNA stability of chloroplasts; J. Nickelsen. G5. Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH); P. Fransz, et al. L4. Tobamovirus transient expression vectors: Tools for plant biology and high-level expression of foreign proteins in plants; G.P.pogue, et al. M1. Identification and characterization of protein interactions using the yeast 2-hybrid system; S.E. Kohalmi, et al. Index. Supplement IV: List of Chapters. The construction of bacterial artificial chromosome (BAC) libraries; S. Choi, R.A. Wing. Yeast as a versatile tool in transcription factor research; A.H. Meijer, et al. Gene transfer mediated by site-specific recombination systems; L.A. Lyznik, et al. Use of the R/RS site-specific recombination system in plants; H. Albert, et al. Cre/Lox directed integration of transgenes into the tobacco genome; H. Albert, D.W. Ow. Plant actin isolation and characterization; C.J. Staiger, et al. Basic Work: Section A: In vitro methods of gene transfer to plant cells. 1. PEG-mediated direct gene transfer and electroporation; R. Bilang, A. Klöti, M. Schrott, I. Potrykus. 2. Gene transfer to plants via particle bombardment; P. Christou. Section B: Agrobacterium-mediated gene transfer to plant cells. 1. Agrobacterium-mediated gene transfer to plant cells: Cointegrate and binary vectors; C.R. Walkerpeach, J. Velten. 2. Specialized vectors for gene tagging and expression studies; C. Koncz, N. Martini, L. Szabados, M. Hrouda, A. Bachmair, J. Schell. 3. Agrobacterium molecular genetics; P.J.J. Hooykaas. 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species; S.B. Gelvin, Chang-Nong Liu. 5. Transient expression assays using GUS constructs and fluorometric detection for
