Methods in Protein Sequence Analysis

1 A Critical Evaluation of Sensitive Amino Acid Analysis.- 1.1 Detection Limits in Amino Acid Analysis: An Overview.- 1.2 Amino Acid Analysis at High Sensitivity: A Critical Review of the Communications.- 1.3 A Comparison of 9-Fluorenylmethylchloroformate (FMOC-Cl) Amino Acid Analysis with Ninhydrin and Phenylthiocarbamyl (PTC) Chemistries.- 1.4 Localization of Endogenous Phosphoserine Residues in the Primary Structure of Proteins.- 1.5 Quantitative N-terminal Analysis of Polypeptides.- 2 New Approaches for the Chemistry of the Edman Degradation.- 2.1 Study on New Edman-type Reagents.- 2.2 Protein Microsequence Analysis with Dansylamino-PITC.- 2.3 Manual Gas Phase Isothiocyanate Degradation.- 3 New Instrumentation in Sequence Analysis and Synthesis of Biopolymers.- 3.1 A Second Generation Solid-phase Protein Sequencer: The Prosequencer™.- 3.2 Development of Membrane Supports for the Solid-phase Sequence Analysis of Proteins and Peptides.- 3.3 Accelerated High Sensitivity Sequence Analysis of Proteins and Peptides Immobilized on Chemically-modified Glass Fiber Discs.- 3.4 A New Modular Sequencer.- 3.5 An Improved Injection System for On-line High Sensitivity Phenylthiohydantoin Amino Acid Analysis.- 3.6 Design and Performance Features of a Simplified Protein Sequencer with an On-Board Isocratic HPLC System.- 3.7 New Aspects in Isocratic HPLC Separation of Phenylthiohydantoin Amino Acids Through the Application of Ionic Detergents.- 3.8 A Procedure for the Manual Precyclization of Fiber Glass Filters used in the ABI 470A Protein Sequencer.- 4 C-terminal Sequence Analysis: Alternative Techniques to the Stepwise Edman Degradation.- 4.1 The Long Search for a Viable Method of C-terminal Sequencing.- 4.2 C-terminal Sequencing: A New Look at the Schlack-Kumpf Thiocyanate Degradation Procedure.- 4.3 Carboxyl-terminal Sequence Analysis of Proteins and Peptides by Chemical Methods.- 4.4 Primary Structure Determination of Proteins by Using a Set of Directly Purified C-terminal Peptides.- 4.5 Immobilized Anhydrotrypsin as a Specific Affinity Adsorbent for Polypeptides Containing Arginyl, Lysyl, or S-Aminoethylcysteinyl Residues at the C-termini.- 4.6 Obtaining Primary Sequence Information from the Carboxy Terminal End of Polypeptides.- 4.7 Reinvestigation on BNPS-Skatole Cleavage of Tryptophan Polypeptides and the C-Terminal Sequencing of the Cleavage Fragments.- 5 New Instrumentation and Approaches in Mass Spectrometry of Biopolymers.- 5.1 Advances in Peptide and Protein Sequencing by High Performance Tandem Mass Spectrometry.- 5.2 Protein Sequence Analysis by Tandem Quadrupole Fourier Transform Mass Spectrometry.- 5.3 Strategy for the Use of Plasma Desorption Mass Spectrometry in Protein Sequence Analysis.- 5.4 Peptide Analysis by Time-of-flight Secondary Ion Mass Spectrometry.- 5.5 Analysis of Post-translational Modification and Processing by High Mass FAB Mass Spectrometry.- 6 HPLC Purification of Proteins and Peptides for Microsequence Analysis; Recovery of Proteins from Gels.- 6.1 NH2-Terminal and Internal Microsequencing of Proteins Electroblotted on Inert Membranes.- 6.2 Initial and Repetitive Yields from Proteins Blotted on PVDF Membranes.- 6.3 Evaluation of Siliconized Glass Fiber Membrane for Protein Chemical Analysis.- 6.4 Rapid Peptide Mapping and Protein Analysis by HPLC.- 6.5 Application of Microbore HPLC for Purifying Proteins and Peptides for Microsequence Analysis.- 6.6 Effect of Bead Size on the Analysis of Proteins and Peptides by High Pressure Liquid Chromatography.- 6.7 The Influence of SDS on Micropreparative Peptide Separations on Reversed Phase Columns.- 6.8 Micro-manipulation and Micro-sequencing of Proteins.- 6.9 Preparative Separation of Bioactive Protein on High Performance Hydrophobic Interaction Chromatography.- 6.10 Cuttelfish Protamines: Amino-acid Sequences of Three Distinct Variants.- 6.11 Direct Characterization of Proteins and Peptides in HPLC by Photodiode Array UV-VIS Detection: A New Approach in the Detection and Characterization of Polypeptides.- 7 New Strategies of Protein and Peptide Characterization.- 7.1 A Fluorescent Labelling Procedure to Facilitate Protein Isolation for Rapid Structural Analysis on a Microscale: Application to Analysis of Post-translational Processing of Newcastle Disease Virus Proteins.- 7.2 Specific Enzymatic Cleavage at Cystine/Cysteine Residues. The Use of ASP-N Endoproteinase.- 7.3 Limited Proteolysis as a Tool to Detect Structure and Dynamic Features of Globular Proteins: Studies on Thermolysin.- 7.4 Biochemical Characterization of Posttranslational Processing Enzymes.- 7.5 Characterization of Gonadotropin Hormone-Releasing Hormone Analogs.- 7.6 Brain Nicotinic Acetylcholine Receptors: A Gene Family.- 7.7 N?-Acetylation of Eukaryotic Proteins: Purification and Characterization of Yeast N?-Acetyltransferase and Acetylcoenzyme A Hydrolase.- 8 Organization of an International Protein DATA Base; Evaluation of Software; Computer-assisted Search and Comparison of Sequences.- 8.1 International Cooperation Among Protein Sequence Data Banks.- 8.2 Current Status of Protein Data Banks.- 8.3 Database Lysis: Computer-assisted Investigation of Cleavage Sites in Proteins.- 9 Protein Folding and Three-dimensional Structure Elucidation of Proteins: Theoretical and Practical Approaches.- 9.1 Preferred Residue Interactions in Protein Subunit and Domain Interfaces.- 9.2 Protein Structural Elements and Folding.- 9.3 Membrane-controlled Folding Mechanism of Hepatitis B Surface Antigen of Human, Woodchuck, Ground Squirrel and Duck.- 9.4 The Three-dimensional Structure of the Pore-forming Fragment of Colicin A.- 9.5 Structural Studies on Crystals of Ribosomal Particles.- 10 Recombinant DNA-Technology as Tool for Protein Sequence and Activity Relationship.- 10.1 Cystatins and Cystatin-like Domains of Multifunctional Proteins: Solid-phase Microsequencing as a Tool to Study Structure-Function Relationships of Protein Inhibitors.- 10.2 New Inhibitory Properties of Eglin C after Specific Mutagenesis.- 10.3 Immuno-chemical Recognition of Synthetic Peptides Based on the Sequence and Three-dimensional Structure of Human Renin: An Immuno-control of Renin Activity.- 10.4 Sequence Directed Design of Recognition Peptides.- 11 Domain Substructure Analysis and Interaction of Biomolecules.- 11.1 The Domain Structures of the E2 Components of the Pyruvate Dehydrogenase Multienzyme Complexes from Bacillusstearothermophilus and Saccharomycescerevisiae.- 11.2 The Identification and Significance of Substructural Domains.- 11.3 Identification of Murine Nuclear Proteins that Bind to the Octamer Control Sequence of Immunoglobulin Genes using 5’-Amino-Oligonucleotides.- 11.4 Structure and Functional Domains of Human Apolipoprotein B-100: A Strategy to Elucidate the Structure Information of a Large Protein.- 12 Immunological Recognition and Strategies.- 12.1 Synthesis of Immunotoxins Using a Temperature Controlled Cross-linking Agent.- 12.2 The Extracellular Functional Sites on the ?-Subunit of Acetylcholine Receptor and of the Receptor-Binding Sites on ?-Neurotoxin.- 12.3 The Antigenic Structure of Lysozyme as Detected by T-cells.- 13 Analysis of Membrane Proteins.- 13.1 Proteinchemical Methods in Use with Complex Membrane Enzymes (Cytochrome C Oxidase).- 13.2 Rhodopsin and G-Protein Linked Receptors.- 13.3 The Cholinergic Binding Site: From Sequence to Function.- 13.4 Characterization of Proteins Involved in Mammalian Mitosis.- Concluding Remarks And Outlook.- Protein Chemistry Renascent.- Protein Chemistry and the Biotechnology of the Future.- Contributors Index.