1 Introduction.- 2 Cytology (With 35 Figures).- 1 Introduction.- 2 The Pellicle.- 3 Cytoplasmic Organelles.- 4 Cytoplasmic Organelle Systems.- 5 Nucleus.- 6 Symbionts.- References.- 3 The Species Concept and Breeding Systems.- 1 Background.- 2 Updating the Taxonomy and Breeding Systems of Paramecium.- 3 Problems and Future Directions.- References.- 4 Mating-Type Inheritance (With 1 Figure).- 1 Introduction.- 2 Genetic Basis of Mating-Type Inheritance.- 3 Macronuclear Differentiations for the Expression of Mating Type.- 4 Genetic Control of Mating-Type Substances.- References.- 5 Conjugation (With 1 Figure).- 1 Introduction.- 2 Induction of Conjugation.- 3 Cell Surface Events in Conjugation.- 4 Nuclear Events in Conjugation.- 5 Perspectives.- References.- 6 Mating-Type Substances.- 1 Introduction.- 2 Role of Mating-Type Substances in Conjugation.- 3 Chemical Nature of the Mating-Type Substances.- 4 Recent Advances in Biochemical and Immunological Studies on the Mating-Type Substances.- 5 Conclusion.- References.- 7 The Cell Cycle and Regulation of Cell Mass and Macronuclear DNA Content (With 10 Figures).- 1 Introduction.- 2 Patterns of Growth, Replication and Morphogenesis.- 3 Control of Cell Cycle Events.- 4 Quantitative Regulation of Cell Components.- 5 Comparison with Other Organisms.- References.- 8 Nuclear Dimorphism and Function.- 1 Introduction.- 2 Nuclear Function During Vegetative Phase.- 3 Nuclear Function During Sexual Cycle.- References.- 9 Aging.- 1 Mortal or Immortal.- 2 Life Cycle Stages.- 3 Fission Life Span or Calendar Life Span.- 4 Nuclear or Cytoplasmic.- 5 Programmed or Error Accumulated.- References.- 10 Organization and Expression of the Nuclear Genome.- 1 Introduction.- 2 Nuclear Dimorphism.- 3 DNA Structure.- 4 Chromatin Structure and Chromosomal Proteins.- 5 Transcription of the Macronuclear Genome.- 6 The Genetic Code.- 7 Conclusions and Summary.- References.- 11 Immobilization Antigens.- 1 Introduction.- 2 Serotypes.- 3 Serotype Transformation.- 4 Localization and Possible Functions.- 5 Chemistry and Molecular Biology of i-Antigens.- 6 Conclusion.- References.- 12 Mitochondria (With 6 Figures).- 1 Introduction.- 2 Genetics.- 3 Respiratory Chain.- 4 Molecular Biology of the Genome.- References.- 13 Electrophysiology (With 4 Figures).- 1 Introduction.- 2 A Historical Note.- 3 Ion Batteries and Membrane Channels.- 4 Properties of the Resting Membrane.- 5 Responses to Stimuli.- 6 Voltage-Dependent Responses.- 7 Topology of Ion Channels.- 8 Conclusion.- References.- 14 Motor Control of Cilia (With 6 Figures).- 1 Introduction.- 2 Galvanotaxis: A Classic Reviewed.- 3 Current Methods.- 4 The Cilium is a Rotary Sliding Machine.- 5 Parameters of Ciliary Activity.- 6 Reactivation of Ciliary Axonemes.- 7 Depolarization-Induced Ciliary Activity (DCA).- 8 Hyperpolarization-Induced Ciliary Activity (HCA).- 9 Adaptation.- 10 Steps in Electromotor Coupling.- 11 Perspectives.- References.- 15 A Genetic Dissection of Ion-Channel Functions (With 8 Figures).- 1 Introduction.- 2 Ion Currents.- 3 Mutants with Defective Currents.- 4 The Use of the Mutants.- 5 A Search for the Gene Products.- 6 Conclusion.- References.- 16 Biochemistry of Cilia (With 5 Figures).- 1 Introduction.- 2 Axenic Mass Culture of Paramecium.- 3 Ciliary Surface Proteins.- 4 Ciliary Lipid Composition.- 5 Ciliary Membrane Proteins.- 6 Axonemal Dynein ATPases.- References.- 17 Behavioral Genetics in P. caudatum (With 2 Figures).- 1 Introduction.- 2 Outline of P. caudatum Genetics and Isolation of Recessive Mutants.- 3 Behavioral Mutants.- 4 Relation of CNRs to Pawns in P. tetraurelia.- 5 Genic and Allelic Interactions of CNR Loci Observed in P. caudatum.- 6 Conclusion.- References.- 18 Chemokinesis (With 7 Figures).- 1 Introduction.- 2 Assays of Chemoresponse.- 3 Swimming behavior.- 4 Stimuli.- 5 Chemoreceptors.- 6 Characteristic Membrane Potential Changes in Chemoresponse: Models for Testing.- 7 Second Messengers.- 8 Summary.- References.- 19 The Lysosome System (With 11 Figures).- 1 Introduction.- 2 Phagosome Formation.- 3 Phagosome Classification.- 4 Phagosome Acidification.- 5 Lysosomal Fusion and Degradation.- 6 Processing or Maturation Period.- 7 Phagosome Defecation.- 8 Membrane Recycling and Replacement.- 9 Concluding Remarks.- References.- 20 Exocytosis: Biogenesis, Transport and Secretion of Trichocysts (With 10 Figures).- 1 Introduction.- 2 Trichocyst Morphology.- 3 Trichocyst Biochemistry.- 4 Biogenesis of the Trichocyst Matrix.- 5 Trichocyst Transport and Docking.- 6 Trichocyst Discharge, Membrane Retrieval and Trichocyst Renewal.- 7 Unsolved Problems and Conclusions.- References.- 21 The Cytoskeleton (With 13 Figures).- 1 Introduction.- 2 Elements of the Cytoskeleton of Paramecium tetraurelia.- 3 General Properties of the Cytoskeleton.- 4 Conclusion.- 5 Technical Note.- References.- 22 Endocytobiosis (With 2 Figures).- 1 Introduction.- 2 Categories of Endocytobiosis.- 3 Establishment of Endocytobionts: Infection and Integration.- 4 Maintenance of Endocytobionts and Adaptations.- 5 Adaptive Value of Endocytobionts.- 6 Conclusions and Perspectives.- References.- 23 Endosymbionts of Killer Paramecia (With 2 Figures).- 1 Introduction.- 2 Taxonomy of Bacterial Endosymbionts of Killer Paramecia.- 3 R Bodies, Extrachromosomal Elements, and Killer Traits.- 4 Kappaphages and R Body-Coding Plasmids.- 5 Genes Required for Type 51 R-Body Synthesis and Their Expression.- 6 Relationships Among R Bodies.- 7 Summary.- References.- 24 Ecology (With 2 Figures).- 1 Introduction.- 2 Distribution.- 3 Experimental Ecology.- 4 Field Ecology.- 5 Research Needs.- References.