Pocket Guide to Biomolecular NMR
Samenvatting
Steering clear of quantum mechanics and product operators, "Pocket Guide to Biomolecular NMR" uses intuitive, concrete analogies to explain the theory required to understand NMR studies on the structure and dynamics of biological macromolecules. For example, instead of explaining nuclear spin with angular momentum equations or Hamiltonians, the books describes nuclei as "bells" in a choir, ringing at specific frequencies depending on the atom type and their surrounding electromagnetic environment.This simple bell analogy, which is employed throughout the book, has never been used to explain NMR and makes it surprisingly easy to learn complex, bewildering NMR concepts, such as dipole-dipole coupling and CPMG pulse sequences. Other topics covered include the basics of multi-dimensional NMR, relaxation theory, and Model Free analysis. The small size and fast pace of “Pocket Guide to Biomolecular NMR” makes the book a perfect companion to traditional biophysics and biochemistry textbooks, but the book's unique perspective will provide even seasoned spectroscopists with new insights and handy “thought” short-cuts.
Specificaties
Inhoudsopgave
1.1 Chemical Choirs
1.2 Essentials of Electromagnetism
1.3 Electromagnetic Microsensors
1.4 Frequency Finders
Mathematical Sidebar 1.1: Fourier Transform
1.5 Basics of one-dimensional NMR
Mathematical Sidebar 1.2 Converting Hz to PPM
References
2 Bonded Bells and Two-Dimensional Spectra
2.1 Introduction to Coupling
2.2 Bonded Bells: J-Coupling
Mathematical Sidebar 2.1: Karplus Equation
2.3 NMR Maps: Two-Dimensional Spectra
Mathematical Sidebar 2.2 Why 12C and 14N atoms are so shy?
2.4 The 1H-15N HSQC: Our Bread and Butter
2.5 Hidden Notes: Creating Two-Dimensional Spectra
References
3 Neighboring Bells and Structure Bundles
3.1 Bumping Bells: Dipole-Dipole Coupling
Mathematical Sidebar 3.1: Dipole-dipole Coupling
3.2 Atomic Meter Stick: the NOE
3.3 Into “Three-D”
3.4 Adult “Connect-the-Dots:” HNCA
3.5 Putting the Pieces Together: A Quick Review
3.6 Wet Noodles and Proteins Bundles: Building a Three-Dimensional Structure
References
4 Relaxation Theory Part One: Silencing of the Bells
4.1 Nothing Rings Forever: Two Paths to Relax
4.2 Relaxation: Ticket to the Protein Prom
Mathematical Sidebar 4.1: Boltzmann Distribution
4.3 Oh-My, How Your Field Fluctuates
4.4 Blowing Off Steam and Returning to Equilibrium: T1
Mathematical Sidebar 4.2: T1 Relaxation
4.5 Loosing Lock-Step : Coherence and T2
Mathematical Sidebar 4.3: T2 Relaxation and Spin Echo
References
5 Relaxation Theory Part Two: Moving Atoms and Changing Notes
5.1 Keeping the Terms Straight
5.2 NMR Dynamics in a Nutshell: The Rules of Exchange
5.3 Two States, One Peak: Atoms in the Fast Lane of Exchange
5.4 Two States, Two Peaks: Atoms in the Slow Lane of Exchange
5.5 Two States, One Strange Peak: Atoms in Intermediate Exchange
5.6 Tumbling Together: Rotational Correlation Time (c)
5.7 Summary
References
6 Protein Dynamics
6.1 Dynamics Analysis by NMR: Multli-Channel Metronomes, Not a GPS
6.2 Elegant Simplicity: Lipari and Szabo Throw Out the Models
6.3 Wagging Tails and Wiggling Bottoms: Local versus Global Motion
6.4 Measuring Fast Motion: Model Free Analysis
Mathematical Sidebar 6.1: Correlation Functions and Model Free
6.5 Changing Directions on the Track: Refocusing Pulses
6.6 Measuring Intermediate Motion: CPMG Relaxation Dispersion Analysis
6.7 Measuring Slow Motion: Z-Exchange Spectroscopy
6.8 Measuring Motion Summary
References