Decellularization Methods of Tissue and Whole Organ in Tissue Engineering
Samenvatting
This contributed volume is the first of a series that introduces safe, feasible, and practical decellularization and recellularization techniques for tissue and organ reconstruction. We have put special emphasis on the research areas most likely to develop well-engineered scaffolds for tissue and organ engineering, while presenting easily applicable bench-to-bedside approaches highlighting the latest technical innovations in the field.
This book includes both a fundamental discussion for a broad understanding of the basis of tissue repair and substitution, as well as chapters written by world renowned specialists from 20 countries providing deeper discussions and analysis of related sub disciplines.
Within these pages, the reader will find state-of-the-art protocols and current clinical challenges in cell and tissue biology, including accurate and comprehensive information on extracellular matrices, natural biomaterials, tissue dynamics, morphogenesis, stem cells, cellular fate progressions, cell and tissue properties for in-vitro and in-vivo applications.
This comprehensive and carefully organized treatise provides a clear framework for graduate students and postdoctoral researchers new to the field, but also for researchers and practitioners looking to expand their knowledge on tissue and organ reconstruction.
Specificaties
Inhoudsopgave
<p>Acknowledgment</p>
<p>Section 1: Introduction of Acellularization</p>
<p>Section 2: Extra Cellular Matrix (ECM) as a Bioscaffold</p>
<p>Section 3: Methods of Characterization of Acellularized Tissue</p>
<p>§ Histology Staining: H&E, Masson's Trichrome Staining, DAPI Staining</p>
<p>§ Determination amount of Hydroxy lysin and Hydroxy Proline</p>
<p>§ DNA Quantification</p>
<p>§ Scanning Electron Microscopy (SEM)</p>
<p>§ Mechanical Strength (Tensile and Compressive test)</p>
<p>§ Sodium Dodecyl Sulfate (SDS) Detection Method</p>
<p>§ Cytotoxicity Assessment : Live/Dead Assay, MTT Assay</p>
<p>§ Cell Attachment and Cell Proliferation</p>
<p>§ CT Angiography </p>
<p>Section 4: Methods of Sterilization of Acellularized Tissue</p>
<p>Section 5: skin</p>
<p>Chapter 1: Thin Skin</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p>Chapter 2: Thick Skin<br></p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p>Section 6: Cartilage Tissue<br></p>
<p>Chapter 1: Hyaline cartilage</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p>Chapter 2: Fibrocartilage<br></p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p>Chapter 3: Elastic cartilage<br></p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 7: Bone Tissue</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 8: Cardiovascular System</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 9: Digestive System I </p>
<p>Chapter 1: Tongue</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 2: Esophagus</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> Chapter 3: Stomach</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 4: Small Intestine</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 5: Large Intestine</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 10: Digestive System II</p>
<p>Chapter 1: Liver</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 2: Pancreases</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding </p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 3: Gallbladder</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> </p>
<p>Section 11: Respiratory System </p>
<p>Chapter 1: Trachea</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 2: Lung</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 12: Urinary System </p>
<p>Chapter 1: Kidney</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Chapter 2: Bladder</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 13: Nerve System </p>
<p>Chapter 1: Central Nerve System (CNS)</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p>Chapter 2: Peripheral Nerve System (PNS)</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 14: Female Reproductive System</p>
<p> Chapter 1: Ovary</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> Chapter 2: Uterus</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> Chapter 3: Vagina</p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> Chapter 4: Cervix</p>
§ History <p></p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p>Section 15: Male Reproductive System</p>
<p>Chapter 1: Prostate Gland</p>
<p>§ History </p>
§ Materials & Methods<p></p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>
<p> </p>
<p> Chapter 2: Testis </p>
<p>§ History </p>
<p>§ Materials & Methods</p>
<p>§ Cell seeding</p>
<p>§ Clinical Applications</p>
<p>§ Limitations</p>
<p>§ Conclusion</p>

