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Plant Epigenetics and Epigenomics

Methods and Protocols

Specificaties
Gebonden, 245 blz. | Engels
Humana Press | 2014e druk, 2014
ISBN13: 9781627037723
Rubricering
Humana Press 2014e druk, 2014 9781627037723
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 8 werkdagen

Samenvatting

Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape. These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.

Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.

Specificaties

ISBN13:9781627037723
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:245
Uitgever:Humana Press
Druk:2014

Inhoudsopgave

<p>1. Landscaping Plant Epigenetics </p><p>Peter C. McKeown and Charles Spillane</p><p> </p><p>2. The Gene Balance Hypothesis: Dosage Effects in Plants</p><p>James A. Birchler<sup> </sup>and Reiner A. Veitia</p><p> </p><p>3. High-throughput RNA-Seq for Allelic or Locus-specific Expression Analysis in Arabidopsis Related Species, Hybrids, and Allotetraploids</p><p>Danny W-K. Ng, Xiaoli Shi, Gyoungju Nah, and Z. Jeffrey Chen</p><p> </p><p>4. Inference of Allele-specific Expression from RNA-seq Data</p><p>Paul K. Korir and Cathal Seoighe</p><p> </p><p>5. Screening for Imprinted Genes using High Resolution Melting Analysis of PCR Amplicons</p><p>Robert Day and Richard Macknight</p><p> </p><p>6. Analysis of Genomic Imprinting by Quantitative Allele-specific Expression by Pyrosequencing®</p><p>Peter C. McKeown, Antoine Fort, and Charles Spillane</p><p> </p><p>7. Endosperm-specific Chromatin Profiling by Fluorescence-activated Nuclei Sorting and Chip-on-chip</p><p>Isabelle Weinhofer and Claudia Köhler</p><p> </p><p>8. Imaging Sexual Reproduction in Arabidopsis using Fluorescent Markers</p><p>Mathieu Ingouff </p><p> </p><p>9. Genome-wide Analysis of DNA Methylation in Arabidopsis using MeDIP-chip</p><p>Sandra Cortijo, René Wardenaar, Maria Colomé-Tatché, Frank Johannes, and Vincent Colot</p><p> </p><p>10. Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-stress Response in Montepulciano and Sangiovese Grape Cultivars</p><p>Emidio Albertini and Gianpiero Marconi</p><p> </p><p>11. Detecting Histone Modifications in Plants</p><p>Jie Song, Bas Rutjens,<sup> </sup>and Caroline Dean</p><p> </p><p>12. Quantitatively Profiling Genome-wide Patterns of Histone Modifications in Arabidopsis thaliana using ChIP-seq </p><p>Chongyuan Luo and Eric Lam</p><p> </p><p>13. Analysis of Retrotransposon Activity in Plants</p><p>Christopher DeFraia and R. Keith Slotkin</p><p> </p><p>14. Detecting Epigenetic Effects of Transposable Elements in Plants </p><p>Christian Parisod, Armel Salmon, Malika Ainouche, and Marie-Angèle Grandbastien</p><p>15. Detection and Investigation of Transitive Gene Silencing Effects in Plants</p><p>Leen Vermeersch, Nancy De Winne, and Ann Depicker<sup></sup></p><p> </p>

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        Plant Epigenetics and Epigenomics