Cereal Genomics

<p> 1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing</p><p>Agnelo Furtado</p><p>2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) </p><p>Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa</p><p> </p><p>3. RNA Extraction from Cereal Vegetative Tissue</p><p>Julie A. Pattemore</p><p> </p><p>4. RNA Extraction from Developing or Mature Wheat Seeds</p><p>Agnelo Furtado</p><p> </p><p>5. cDNA Library Preparation</p><p>Maarten Kooiker and Gang-Ping Xue</p><p> </p><p>6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants</p><p>Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining</p><p> </p><p>7. The Polymerase Chain Reaction (PCR) - General Methods</p><p>Daniel L. E. Waters and Frances M. Shapter</p><p> </p><p>8. Mutation and Mutation Screening</p><p>L. Slade Lee, Bradley J. Till,  Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak</p><p> </p><p>9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression</p><p>Timothy L. Fitzgerald and Richard B. McQualter</p><p> </p><p>10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel  </p><p>Agnelo Furtado</p><p>11. Rapid Cloning of Genes and Promoters for Functional Analyses</p><p>Peer M. Schenk</p><p> </p><p>12. Genome Walking</p><p>Frances M. Shapter and Daniel L.E. Waters</p><p> </p><p>13. Functional Analysis by Protein Biochemistry</p><p>Louis M. T. Bradbury</p><p> </p><p>14. Genomic Southern Blot Analysis</p><p>Leigh Gebbie</p><p> </p><p>15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs </p><p>Shazia Iram</p><p>16. Protein Blotting-Protocol for Beginners</p><p>Lars A. Petrasovits</p><p> </p><p>17. Genetic Transformation of Wheat via Particle Bombardment</p><p>Caroline A. Sparks &amp; Huw D. Jones</p><p> </p><p>18. Sorghum Genetic Transformation by Particle Bombardment </p><p>Guoquan Liu, Bradley C. Campbell and Ian D. Godwin</p><p> </p><p>19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery</p><p>Caroline A. Sparks, Angela Doherty &amp; Huw D. Jones</p><p> </p><p>20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation </p><p>Wendy A Harwood</p><p> </p><p>21. Agrobacterium-mediated Transformation- Rice Transformation</p><p>Inez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo </p> </p><p>22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos</p><p>Hyeyoung Lee and Zhanyuan J. Zhang</p><p> </p><p>23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops</p><p>Zining Wang</p><p> </p><p>24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data</p><p>Gopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry</p><p> </p><p>