Plant Transposable Elements

Methods and Protocols

Specificaties
Gebonden, 324 blz. | Engels
Humana Press | 2013e druk, 2013
ISBN13: 9781627035675
Rubricering
Humana Press 2013e druk, 2013 9781627035675
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

Transposable elements have played a major role in shaping plant genome structure and gene expression.  Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome.  In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.

Specificaties

ISBN13:9781627035675
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:324
Uitgever:Humana Press
Druk:2013

Inhoudsopgave

<p>1. Historical Overview of Transposable Element Research </p><p>Peter A Peterson</p><p> </p><p>2. Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities</p><p>Virginia Walbot</p><p> </p><p>3. Using Transposons for Genetic Mosaic Analysis of Plant Development</p><p>Philip W. Becraft</p><p> </p><p>4. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity</p><p>Lakshminarasimhan Krishnaswamy, Thomas Peterson</p><p> </p><p>5. Molecular Biology of Maize Ac/Ds Elements – an Overview</p><p>Katina Lazarow, My-Linh Du and Reinhard Kunze</p><p> </p><p>6. Gene Tagging with Engineered Ds Elements in Maize</p><p>Yubin Li, Gregorio Segal, Qinghua Wang, and Hugo K. Dooner</p><p> </p><p>7. Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice </p><p>Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun, Doh Won Yun, Gang-Seob Lee, Tae Ho Kim, Chang-deok Han</p><p> </p><p>8. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting</p><p>Dafang Wang and Thomas Peterson</p><p> </p><p>9. Regulation of the Mutator System of Transposons in Maize</p><p>Damon Lisch </p><p> </p><p>10. Using MuDR/Mu Transposons in Directed Tagging Strategies  </p><p>Virginia Walbot and Julia Qüesta</p><p> </p><p>11. Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines</p><p>Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Wayne T. Avigne, and Karen E. Koch</p><p> </p><p>12. Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning</p><p>Sanzhen Liu, An-Ping Hsia, Patrick S. Schnable</p><p> </p><p>13. Molecular Genetics and Epigenetics of CACTA Elements</p><p>Nina V. Fedoroff</p><p> </p><p>14. Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis</p><p>Amal Harb and Andy Pereira</p>15. Reverse Genetics in Rice using Tos17<p>Delphine Mieulet, Anne Diévart, Gaëtan Droc, Nadège Lanau and Emmanuel Guiderdoni</p><p> </p>16. Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System </p><p>Tom Gerats , Jan Zethof, and Michiel Vandenbussche</p><p> </p><p>17. Transposon Display: A Versatile Method for Transposon Tagging</p><p>Michiel Vandenbussche, Jan Zethof, and Tom Gerats</p><p> </p><p>18. Massive Indexed Parallel Identification of Transposon Flanking Sequences</p><p>Michiel Vandenbussche, Jan Zethof, and Tom Gerats</p><p> </p><p>19. Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition</p><p>Moaine Elbaidouri, Cristian Chaparro and Olivier Panaud </p><p> </p><p>20. Overview of Repeat Annotation and de novo Repeat Identification</p><p>Ning Jiang</p><p> </p><p>21. Computational Methods for Identification of DNA Transposons</p><p>Ning Jiang</p><p> </p><p>22. TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements</p><p>Brent A. Kronmiller and Roger P. Wise<sup></sup></p><p> </p>

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        Plant Transposable Elements