<p>1. PPARs: History and Advances</p><p> Jihan Youssef and Mostafa Badr </p><p> </p><p>2. PPAR-Alpha Cloning, Expression and Characterization</p><p> Suong N. T. Ngo and Ross McKinnon</p><p> </p><p>3. PPARGC1A and PPARG Genotyping. Beginner’s Guide to Genotyping with Unlabeled Probes</p><p> Viera Habalova and Josef Židzik</p><p> </p><p>4. Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARβ/d Overexpression</p><p>Teayoum Kim, Olga Zhelyabovska, Jian Liu, and Qinglin Yang</p><p> </p><p>5. Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering</p><p> Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun</p><p> </p><p>6. Dominant-Negative and Knockdown Approaches to Studying PPAR activity</p><p> Sudhir Singh and Robert G. Bennett</p><p> </p><p>7. Producing PPARgamma2 Knockdown in Mouse Liver</p><p> Tomomi Yamazaki and Osamu Ezaki</p><p> </p><p>8. Adipose Tissue-Specific PPARg Gene Targeting</p><p> Weimin He</p><p> </p><p>9. Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs</p><p> Nico Mitro, Frederica Gilardi, Marco Giudici, Cristina Godio, Elena Scotti, and Maurizio Crestani</p><p> </p><p>10. PPAR SUMOylation: Some Useful Experimental Tips</p><p> Benoit Pourcet, Bart Staels, and Corine Glineur</p><p> </p><p>11. Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a</p><p> Ji Suk Chang and Thomas W. Gettys</p><p> </p><p>12. In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses</p><p> Till Adhikary and Rolf Müller</p><p> </p><p>13. FISH Analysis Using PPARg Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms</p><p> Sharifah Noor Akmal Syed Hussain, Chia Wai Kit, and Zubaidah Zakaraia</p><p> </p><p>14. Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections</p><p> Carolyn M. Komar and Mary Jane Long</p><p> </p><p>15. Determination of PPAR Expression by Western-Blot</p><p> Aziz Gullich and Catherine Coirault</p><p> </p><p>16. Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators</p><p> Nico Mitro, Cristina Godio, and Maurizio Crestani</p><p> </p><p>17. Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach</p><p> Fabio Lannutti, Alessandro Marrone, and Nazzareno Re</p><p> </p><p>18. Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg</p><p> Silvia Zorrilla and Dolores Pérez-Sala</p><p> </p><p>19. Exploring PPAR Modulation in Experimental Mice</p><p> Anna Rull, Gerard Aragonès, Raúl Beltrán-Debón, Esther Rodríguez-Gallego, Jordi Camps, Jorge Joven</p><p> </p><p>20. Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold</p><p> Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa</p><p> </p><p>21. Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry</p><p> Mathias Muller, Christian Ihling, and Andrea Sinz</p><p> </p><p>22. Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites – Targets in Sports Drug Testing</p><p> Mario Thevis, Ines Möller, Simon Beuk, and Wihelm Schänzer</p><p> </p><p>23. LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPARg Agonist KR-62980</p><p> Mi-Sun Kim and Myung Ae Bae</p><p> </p><p>24. Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury</p><p> Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins</p>
