Ubiquitin-Proteasome Protocols

Biochemical Methods to Study the Ubiquitin-Proteasome System.- Small-Molecule Inhibitors of Proteasome Activity.- Purification of E1 and E1-Like Enzymes.- Assays for RING Family Ubiquitin Ligases.- Ubiquitin Chain Synthesis.- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast.- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome.- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation.- Assays of Proteasome-Dependent Cleavage Products.- Identification of Components of Protein Complexes.- Mass Spectrometric Determination of Protein Ubiquitination.- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol.- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation.- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization.- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions.- Measuring Ubiquitin Conjugation in Cells.- Assays for Proteasome Assembly and Maturation.- N-Terminal Ubiquitination.- Quantitating Defective Ribosome Products.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells.- Aggresome Formation.- Detection of Sumoylated Proteins.- Proteasome Inhibitors in Cancer Therapy.- Parkinson’s Disease.