Genetics in Vascular Disease.- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis.- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis.- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes.- Isolation of Genes Expressed in Vasular Tissue.- Efficient Extraction of RNA from Vascular Tissue.- Preparation of cDNA Libraries from Vascular Cells.- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells.- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR.- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques.- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells.- Identification of Novel Protein Kinases in Vascular Cells.- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells.- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes.- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells.- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells.- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells.- Molecular Analysis of MRNA Expression in Vascular Cells.- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis.- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases.- Localization of Gene Expression inHuman Atherosclerotic Lesions by In SituHybridization.- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue.- Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus.- Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL).- In Vitro Detection of Apoptosis in Isolated Vascular Cells.- Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis.- In Vitro Methods to Express Foreign Genes in Vasular Cells.- Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells.- Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells.- Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells.- High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection.- Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Marker.- Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells.- Embryonal Stem (ES) Cell-Derived Macrophages.- Delivery of Recombinant Adenoviruses to Human Saphenous Vein.- In Vivo Vascular Gene Transfer Protocols.- An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization.- Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo.- Gene Delivery to Rabbit Arteries Using the Collar Model.- Delivery of Antisense Oligonucleotides to the Vascular Wall.- Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo.
