Cereal Genomics

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer US | e druk, 2019
ISBN13: 9781493998647
Rubricering
Springer US e druk, 2019 9781493998647
Onderdeel van serie Methods in Molecular Biology
€ 216,99
Levertijd ongeveer 8 werkdagen

Samenvatting

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

Specificaties

ISBN13:9781493998647
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

Inhoudsopgave

<p>A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.-&nbsp;Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.-&nbsp;Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.-&nbsp;Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.-&nbsp;Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing.-&nbsp;Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.-&nbsp;DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.-&nbsp;Genome-wide Identification of Regulatory DNA Elements in Crop Plants.-&nbsp;Genome-wide Profiling of Histone Modifications with ChIP-seq.-&nbsp;Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.-&nbsp;Genome-wide Identification of Allele-Specific Gene Expression in a Parent‑of‑Origin Specific Manner.-&nbsp;Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).-&nbsp;Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.-&nbsp;Emerging Genome Engineering Tools in Crop Research and Breeding.-&nbsp;CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.-&nbsp;Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne.-&nbsp;Agrobacterium Transformation in the Rice Genome.-&nbsp;In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C<sub>4</sub> Photosynthesis in Zea mays.</p>

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Levertijd ongeveer 8 werkdagen

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        Cereal Genomics