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Enzyme-Mediated Ligation Methods

Specificaties
Paperback, blz. | Engels
Springer New York | e druk, 2020
ISBN13: 9781493995486
Rubricering
Springer New York e druk, 2020 9781493995486
Onderdeel van serie Methods in Molecular Biology
€ 138,99
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Samenvatting

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. 

Specificaties

ISBN13:9781493995486
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

Inhoudsopgave

<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>1. Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates</div><div>Rémy Gébleux, Manfred Briendl, Ulf Grawunder, and Roger R. Beerli</div><div><br></div><div>2. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)</div><div>Hejia Henry Wang and Andrew Tsourkas</div><div><br></div><div>3. Cyclizing Disulfide-Rich Peptides using Sortase A</div><div>Akello J. Agwa, David J. Craik, and Christina I. Schroeder</div><div><br></div><div>4. Chemo-Enzymatic Synthesis of&nbsp; Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1</div><div>Marcel Schmidt and Timo Nuijens</div><div><br></div><div>5. Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)</div><div>Igor Rupniewski and David Rabuka</div><div><br></div>6. Butelase 1-Mediated Ligation of Peptides and Proteins<div>Xinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, and James P. Tam</div><div><br></div><div>7. Trypsiligase-Catalyzed Peptide and Protein Ligation</div><div>Sandra Liebscher and Frank Bordusa</div><div><br></div><div>8. Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase</div><div>Stephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, and Nicolas Rasche</div><div><br></div><div>9. Tailoring Activity and Selectivity of Microbial Transglutaminase&nbsp;</div><div>Lukas Deweid, Olga Avrutina, and Harald Kolmar</div><div><br></div>10. SpyLigase-Catalyzed Modification of Antibodies<div>Vanessa Siegmund, Birgit Piater, Frank Fischer, and Harald Kolmar</div><div><br></div><div>11. Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases</div><div>Wael E. Houssen</div><div><br></div><div>12. In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis</div><div>Karen S. Harris, Simon Poon, Pedro Quimbar, and Marilyn A. Anderson</div><div><br></div><div>13. Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation</div><div>Jan Grünewald, Ansgar Brock, and Bernhard H. Geierstanger</div><div><br></div>14. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization<div>Joseph G. Plaks and Joel L. Kaar</div><div><br></div><div>15. BioID as a Tool for Protein Proximity Labeling in Living Cells</div><div>Rhiannon M. Sears, Danielle G. May, and Kyle J. Roux</div><div><br></div>16. N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells<div>Samuel H. Ho and David A. Tirrell</div><div><br></div><div>17. Tubulin Tyrosine Ligase-Mediated Modification of Proteins</div><div>Marcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P.R. Hackenberger, Dominik Schumacher, and Jonas Helma</div><div><br></div><div>18. Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine</div><div>Jorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, and Floria L. van Delft</div><div><br></div>
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