<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>PART I DETECTION OF LIPID MODIFICATIONS<br><br></div><div>1. Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods</div><div> Charlotte H. Hurst, Dionne Trumbull, and Piers A. Hemsley</div><div><br></div><div>2. Detection of Heterogeneous Protein S-Acylation in Cells</div><div> Jennifer Greaves and Nicholas C. O. Tomkinson</div><div><br></div><div>3. Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes</div><div> Mina Ahmadi, Kiall Francis Suazo, and Mark D. Distefano</div><div><br></div><div>4. Chemical Proteomic Analysis of S-Fatty Acylated Proteins and their Modification Sites</div><div> Emmanuelle Thinon and Howard C. Hang</div><div> </div><div>5. Direct Analysis of Protein S-Acylation by Mass Spectrometry</div> Yuhaun Ji and Cheng Lin<div><br></div><div>6. Enrichment of S-Palmitoylated Proteins for Mass Spectrometry Analysis</div><div> Melanie Cheung See Kit and Brent R. Martin</div><div><br></div><div>PART II REVERSIBLE FATTY ACYLATION<br><br></div><div>7. Systematic Screening of Depalmitoylating Enzymes and Evaluation of their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay</div><div> Takashi Kanadome, Norihiko Yokoi, Yuko Fukata, and Masaki Fukata</div><div><br></div><div>8. Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes</div><div> Rahul S. Kathayat and Bryan C. Dickinson</div><div><br></div><div>9. Dynamic Radiolabeling of S-Palmitoylated Proteins</div><div> Laurence Abrami, Robin A. Denhardt-Eriksson, Vassily Hatzimanikatis, and F. Gisou van der Goot</div><div><br></div><div>10. Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins</div><div> Jun Young Hong, Ji Cao, and Hening Lin</div><div> </div><div>11. Global Profiling of Sirtuin Deacylase Substrates Using Chemical Proteomic Strategy and Validation by Fluorescent Labeling</div><div> Shuai Zhang, Nicole A. Spiegelman, and Hening Lin</div><div><br></div><div>PART III zDHHC S-ACYLTRANSFERASES</div><br><div>12. siRNA Knockdown of Mammalian zDHHCs and Validation of mRNA Expression by RT- qPCR</div><div> Heather McClafferty and Michael J. Shipston</div><div><br></div><div>13. In Vitro Assays to Monitor the Enzymatic Activities of zDHHC Protein Acyltransferases</div><div> David A. Mitchell, Laura C. Pendleton, and Robert J. Deschenes</div><div><br></div><div>14. Purification of Recombinant zDHHC Proteins Using an Insect Cell Expression System</div><div> Martin Ian P. Malgapo and Maurine E. Linder</div><div><br></div><div>15. Bioinformatic Identification of Functionally and Structurally Relevant Residues and Motifs in Protein S-Acyltransferases</div><div> Rodrigo Quiroga and Javier Valdez Taubas</div><div><br></div><div>PART IV ONLINE RESOURCE<br><br></div><div>16. SwissPalm 2: Protein S-Palmitoylation Database</div><div> Mathieu Blanc, Fabrice P. A. David, and F. Gisou van der Goot</div><div><br></div><div>PART V FATTY ACYLATION IN THE LUMEN OF THE SECRETORY PATHWAY</div><div><br>17. Probing Interaction of Lipid-Modified Wnt Protein and Its Receptors by ELISA</div><div> Aaron H. Nile and Rami N. Hannoush</div><div><br></div><div>18. Biochemical Assays for Ghrelin Acylation and Inhibition of Ghrelin O-Acyltransferase</div> Michelle A. Sieburg, Elizabeth R. Cleverdon, and James L. Hougland<div><br></div><div>19. In Vitro Analysis of Hedgehog Acyltransferase and Porcupine Fatty Acyltransferase Activities </div><div> James John Asciolla, Kalpana Rajanala, and Marilyn D. Resh</div><div><br></div><div>PART VI PRENYLATION AND POST-PRENYLATION PROCESSING </div><div><br>20. Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System</div><div> William Gillette, Peter Frank, Shelley Perkins, Matthew Drew, Carissa Grose, and Dominic Esposito</div><div><br></div><div>21. A Quantitative FRET Assay for the Upstream Cleavage Activity of the Integral Membrane Proteases Human ZMPSTE24 and Yeast Ste24</div><div> Erh-Ting Hsu, Jeffrey S. Vervacke, Mark D. Distefano, and Christine A. Hrycyna</div><div><br></div><div>PART VII BIOCHEMISTRY OF PROTEIN LIPIDATION<br><br></div><div>22. Monitoring RhoGDI Extraction of Lipid-Modified Rho GTPases from Membranes using Click Chemistry</div><div> Akiyuki Nishimura and Maurine E. Linder</div><br><div>23. Purification of the Rhodopsin-Transducin Complex for Structural Studies</div><div> Yang Gao, Jon W. Erickson, Richard A. Cerione, and Sekar Ramanchandran</div><div><br></div><div>24. Reconstitution of the Rhodopsin-Transducin Complex into Lipid Nanodiscs</div><div> Yang Gao, Jon W. Erickson, Richard A. Cerione, and Sekar Ramanchandran</div><div><br></div>
