High Throughput Screening
Methods and Protocols
Samenvatting
This third edition volume
expands on the previous editions both by presenting more detailed protocols for
the techniques described in the first and second editions of High Throughput
Screening: Methods and Protocols
and by covering important new procedures. The first chapter of
this book provides an overview of important assay development techniques, while
the rest of the chapters detail how to develop and execute screens at whatever
throughput the user needs. Some chapter examples are: structure-based virtual
screening, high throughput screening using mass spectrometry, identification of state-dependent
blockers for voltage gated calcium channels, bioluminescence resonance
energy transfer platform to monitor protein-protein interactions in live cells,
high throughput flow cytometry, and application of imaging-based assays in microplate formats for high
content screening. Written in the highly successful Methods in Molecular
Biology series format, chaptersinclude introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.
Cutting edge and thorough, High Throughput Screening: Methods and Protocols,
Third Edition, is a valuable resource for anyone who is interested in HTS
research.
Specificaties
Inhoudsopgave
Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method.- High Throughput Screening Using
Mass Spectrometry within Drug Discovery.- Structure-Based Virtual Screening
of Commercially Available Compound Libraries.- AlphaScreen-Based Assays:
Ultra-High Throughput Screening for Small Molecule Inhibitors of Challenging
Enzymes and Protein-Protein Interactions.- Instrument Quality Control.- Application of Fluorescence
Polarization in HTS Assays.- Time Resolved Fluorescence Assays.- Protein Kinase Selectivity
Profiling Using Microfluid Mobility Shift Assays.- Screening for Inhibitors of
Kinase Autophosphorylation.- A Fluorescence-Based High
Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic
Fungus Aspergillus fumigatus.- Mycobacterium tuberculosis
High Throughput Screening.- Identification of
State-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR Based
Assay.- A Luciferase Reporter Gene
System for High Throughput Screening of γ-Globin Gene
Activators.- A High Throughput Flow Cytometry
Assay for Identification of Inhibitors of 3’, 5’-Cyclic Adenosine Monophosphate
Efflux.- High Throughput Cell Toxicity
Assays.- BRET<sup>n</sup>: NanoLuc-Based
Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein
Interactions in Live Cells.- Application of Imaging-Based
Assays in Microplate Formats for High Content Screening.<br></p>