Single Molecule Analysis

Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions.- RNA Unzipping and Force Measurements with a Dual Optical Trap.- Protein Tethering for Folding Studies.- Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein.- A Brief Introduction to Single-Molecule Fluorescence Methods.- Fluorescent Labeling of Proteins.- Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins.- Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans.- Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy.- Fluorescence Microscopy of Nanochannel-Confined DNA.- Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin--Microtubule Complexes.- Single Molecule FRET Analysis of DNA Binding Proteins.- Atomic Force Microscopy: An Introduction.- Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy.- Atomic Force Microscopyof Protein Shells: Virus Capsids and Beyond.- Combined Magnetic Tweezers and Micro-Mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization.- Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions.- Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS).- Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy.