The Renin-Angiotensin-Aldosterone System

<p>A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques.-&nbsp;A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining.-&nbsp;Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches.-&nbsp;Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells.-&nbsp;Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta.-&nbsp;Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice.-&nbsp;Blood Pressure Monitoring using Radio Telemetry Method in Mice.-&nbsp;Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.-&nbsp;Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria.-&nbsp;Comprehensive Assessments of Energy Balance in Mice.-&nbsp;In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis.-&nbsp;A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt.-&nbsp;Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors.-&nbsp;A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection.-&nbsp;Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry.</p>