<p>1. The Phytoplasmas - An Introduction</p><p> Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts </p><p> </p><p>2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus</p><p> Jennifer Hodgetts, David Crossley, and Matt Dickinson</p><p> </p><p>3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture</p><p> Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo </p><p> </p><p>4. Dodder Transmission of Phytoplasmas</p><p>Jaroslava Přibylová and Josef Špak</p><p> </p><p>5. Insect Maintenance and Transmission</p><p> Heather Kingdom</p><p> </p><p>6. Capturing Insect Vectors of Phytoplasmas</p><p> Phyllis Weintraub and Jürgen Gross</p><p> </p><p>7. Insect Vector Transmission Assays</p><p> Domenico Bosco and Rosemarie Tedeschi</p><p> </p><p>8. Molecular Identification of Phytoplasma Vector Species</p><p> Sabrina Bertin and Domenico Bosco </p><p> </p><p>9. Dienes’ Staining and Light Microscopy for Phytoplasma Visualization</p><p> Rita Musetti </p><p> </p><p>10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas</p><p> Nancy S. Andrade and Nolberto S. Arismendi </p><p> </p><p>11. Visualisation of Phytoplasmas using Electron Microscopy</p><p> B. Jean Devonshire</p><p> </p><p>12. Automated DNA Extraction for Large Numbers of Plant Samples</p><p> Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia</p><p> </p><p>13. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts</p><p> Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick </p><p> </p><p>14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene </p><p> Bojan Duduk, Samantha Paltrinieri, Ing Ming Lee, and Assunta Bertaccini</p><p> </p><p>15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon</p><p> Marta Martini and Ing-Ming Lee</p><p> </p><p>16. Tuf and secY PCR Amplification and Genotyping of Phytoplasmas</p><p> Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová, and Milan Navrátil</p><p> </p><p>17. PCR Analysis of Phytoplasmas Based on the secA Gene</p><p> Matt Dickinson and Jennifer Hodgetts</p><p> </p><p>18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains</p><p> Martina Šeruga Musić and Dijana Škorić</p><p> </p><p>19. Microarrays for Universal Detection and Identification of Phytoplasmas</p><p> Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccine</p><p> </p><p>20. T-RFLP for Detection and Identification of Phytoplasmas in Plants</p><p> Jennifer Hodgetts and Matt Dickinson</p><p> </p><p>21. Real-Time PCR for Universal Phytoplasma Detection and Quantification </p><p> Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen</p><p> </p><p>22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA</p><p> Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia</p><p> </p><p>23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group</p><p> Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, and Marina Dermastia</p><p> </p><p>24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions</p><p> Paola Margaria and Sabrina Palmano</p><p> </p><p>25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)</p><p> Jenny Tomlinson </p><p> </p><p>26. DNA Bar-Coding for Phytoplasma Identification</p><p> Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen</p><p> </p><p>27. Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison</p><p> Hiromi Nishida</p><p> </p><p>28. The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment</p><p> Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis</p><p> </p><p>29. Phytoplasma Proteomic Analysis</p><p> Xianling Ji and Yingping Gai</p><p> </p><p>30. Preparation of Phytoplasma Membrane Recombinant Proteins</p><p> Luciana Galetto, Majid Siampour, and Cristina Marzachì</p><p> </p><p>31. Phytoplasma Plasmid DNA Extraction</p><p> Mark T. Andersen and Lia W. Liefting</p><p> </p><p>32. Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA</p><p> Lucy T.T. Tran-Nguyen and Bernd Schneider</p><p> </p><p>33. Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants</p><p> Carmine Marcone</p><p> </p><p>34. Mapping the Phytoplasma Chromosome</p><p> Sylvie Malembic-Maher and Patricia Carle</p>
