<p>Part I: Background and Overview</p> <p> </p> <p>1. Structural Biology of Nonribosomal Peptide Synthetases</p> <p> Bradley R. Miller and Andrew M. Gulick</p> <p> </p> <p>2. The Assembly Line Enzymology of Polyketide Biosynthesis</p> <p> Marisa Till and Paul R. Race</p> <p> </p> <p>Part II: In Vitro Methods</p> <p> </p> <p>3. Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay</p> <p> Benjamin P. Duckworth, Daniel J. Wilson, and Courtney C. Aldrich</p> <p> </p> <p>4. Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains</p> <p> Fumihiro Ishikawa and Hideaki Kakeya</p> <p></p> <p></p> <p>5. Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases</p> <p> Chitose Maruyama, Haruka Niikura, Masahiro Takakuwa, Hajime Katano, and Yoshimitsu Hamano</p> <p> </p> <p>6. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis</p> <p> Clara Brieke, Veronika Kratzig, Madeleine Peschke, and Max J. Cryle</p> <p> </p> <p>7. Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro</p> <p> Ralph A. Cacho and Yi Tang</p> <p> </p> <p>8. The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis</p> <p> Haoran Zhang, Lei Fang, Marcia S. Osburne, and Blaine A. Pfeifer</p> <p> </p> <p>9. Screening for Expressed Non</p>ribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics<p></p> <p> Yunqiu Chen, Ryan A. McClure, and Neil L. Kelleher</p> <p> </p> <p>10. Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi</p> <p> Alexandra A. Soukup, Nancy P. Keller, and Philipp Wiemann</p> <p> </p> <p>11. In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging</p> <p> Delphine Debois, Marc Ongena, Hélène Cawoy, and Edwin De Pauw</p> <p> </p> <p>12. Secondary Metabolic Pathway-Targeted Metabolomics</p> <p> Maria I. Vizcaino and Jason M. Crawford</p> <p> </p> <p>Part III: Bioinformatic Methods</p> <p> </p> <p>13. Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra</p> < Timo Horst Johannes Niedermeyer<p></p> <p> </p> <p>14. Bioinformatics Tools for the Discovery of New Nonribosomal Peptides</p> <p> Valérie Leclère, Tilmann Weber, Philippe Jacques, and Maude Pupin</p> <p> </p> <p>15. The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways</p> <p> Nicolas Tremblay, Patrick Hill, Kyle R. Conway, and Christopher N. Boddy</p> <p> </p> <p>16. Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains</p> <p> Guillermin Agüero-Chapin, Gisselle Pérez-Machado, Aminael Sánchez-Rodríguez, Miguel Machado Santos, and Agostinho Antunes</p> <p> </p> <p>17. Characterization of Nonribosomal Peptide Synthetases with NRPSsp</p> <p> C</p>arlos
Prieto</p>
