Javier Martín
Springer New York
e druk, 2016
9781493932917
Poliovirus
Methods and Protocols
Specificaties
Gebonden, blz.
|
Engels
Springer New York |
e druk, 2016
ISBN13: 9781493932917
Rubricering
Onderdeel van serie
Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Specificaties
ISBN13:9781493932917
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York
Inhoudsopgave
<p>An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication.- Poliovirus Laboratory Based Surveillance: An Overview.- Isolation and Characterisation of Enteroviruses from Clinical Samples.- Isolation and Characterisation of Poliovirus in Cell Culture Systems.- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol.- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays.- A Transgenic Mouse Model of Poliomyelitis.- Standardized Methods for Detection of Poliovirus Antibodies.- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR.- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative.- Phylogenetic Analysis of Poliovirus Sequences.- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA.- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses.- Methods to Monitor Molecular Consistency of Oral Polio Vaccine.- Methods for the Quality Control of Inactivated Poliovirus Vaccines.- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.- Identification and Analysis of Antiviral Compounds against Poliovirus. </p><p></p><p></p>