P-Type ATPases

<p>An Introduction to P-type ATPase research.- &nbsp;Purification of Na,K-ATPase from Pig Kidney.- Preparation of Ca²⁺-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum.- Isolation of H⁺/K⁺-ATPase-enriched Membrane Fraction from Pig Stomachs.- Overproduction of PiB-type ATPases.- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.- The Plasma Membrane Ca²⁺-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein.- Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System.- Time-dependent Protein Thermostability Assay.- Colorimetric Assays of Na,K-ATPase.- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay.- Antimony-Phosphomolybdate ATPase Assay.- ATPase Activity Measurements using radiolabeled ATP.- Assaying P-type ATPases Reconstituted in Liposomes.- Coupling ratio for Ca²⁺ Transport by Calcium Oxalate Precipitation.- Calcium Uptake in Crude Tissue Preparation.- Measuring H⁺ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems.- Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives.- The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases.- Phosphorylation/Dephosphorylation Assays.- Tryptophan Fluorescence Changes Related to Ca²⁺-ATPase Function.- Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca²⁺-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling.</p><p></p><p></p><p>23.&nbsp; Ca²⁺ Binding and Transport Studied with Ca²⁺/EGTA Buffers and ⁴⁵Ca²⁺</p><p></p><p>24.&nbsp; Assay of Copper Transfer and Binding to P_1B-ATPases</p><p></p><p>&nbsp;</p><p></p><p>25.&nbsp; Voltage Clamp Fluorometry of P-Type ATPases</p><p></p><p>26.&nbsp; Electrophysiological Measurements on Solid Supported Membranes </p><p></p><p>27.&nbsp; Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping</p><p>&nbsp;</p><p>&nbsp;</p><p></p>28.&nbsp; Functional Studies of Na⁺,K⁺-ATPase using Transfected Cell Cultures<p></p><p></p><p>29.&nbsp; HPLC Neurotransmitter Analysis</p><p>&nbsp;</p><p>30.&nbsp; Behavior Test Relevant to a₂/a₃Na⁺/K⁺-ATPase Gene Modified Mouse Models</p><p>&nbsp;</p><p>31.&nbsp; Zebrafish Whole-mount in situ Hybridization Followed by Sectioning</p><p>&nbsp;</p><p>32.&nbsp; Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli</p><p></p><p>33.&nbsp; Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives</p><p>&nbsp;</p>34.&nbsp; Transient Expression of P-type ATPases in Tobacco Epidermal Cells<p></p><p></p><p>&nbsp;</p><p></p><p>35.&nbsp; Lipid Exchange by Ultracentrifugation</p><p></p><p>36.&nbsp; Reconstitution of Na⁺, K⁺-ATPase in Nanodiscs</p><p></p><p></p>37.&nbsp; Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method<p></p><p></p><p>38.&nbsp; Two-dimensional Crystallization of the Ca²⁺-ATPase for Electron Crystallography</p><p>39.&nbsp; Two-dimensional&nbsp; Crystallization&nbsp; of&nbsp; Gastric&nbsp; H+,K+-ATPase&nbsp; for&nbsp; Structural&nbsp; Analysis&nbsp; By Electron Crystallography</p><p></p><p></p><p>40.&nbsp; MD Simulations of P-type ATPases in a Lipid Bilayer System</p><p>41.&nbsp; Computational Classification of P-Type ATPases</p><p>&nbsp;</p><p>42.&nbsp; Molecular Modeling of Fluorescent SERCA Biosensors</p><p>&nbsp;</p><p>43.&nbsp; How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example</p><p>Jesper L</p><p>&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p><p>&nbsp;</p><p></p>