<p></p><p>Part I Introduction</p><p>1. Perspectives and Pitfalls in Nucleic Acids Crystallography<br>Eric Westof</p><p>Part II RNA Preparation and crystallogenesis</p><p>2. Preparation of Short 5’-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies<br>Nikita Vasilyev and Alexander Serganov</p><p> 3. Preparation and Crystallization of Riboswitches<br>Alla Peselis, Ang Gao, and Alexander Serganov<br><br></p><p>4. In Vitro/In Vivo Production of tRNA for X-Ray Studies<br>Clément Dégut, Alexandre Monod, Franck Brachet, Thibaut Crépin, and Carine Tisné<br></p><p>5. Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA<br>Mélanie Meyer and Benoît Masquida<br><br></p>6. Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs<br>Adrian R. Ferré-D’Amaré<br><br><p><p>7. Fab Chaperone-Assisted RNA Crystallography (Fab CARC)<br>Eileen Sherman, Jennifer Archer, and Jing-Dong Ye<br><br></p><p>8. Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes<br>Martin Egli and Pradeep S. Pallan</p><p> 9. Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes<br>Cyrielle da Veiga, Joelle Mezher, Philippe Dumas, and Eric Ennifar</p><p> Part III Data Collection, Phasing and Refinement</p><p>10. Crystallographic Data and Model Quality<br>Kay Diederichs<br></p><p>11. Advanced Crystallographic Data Collection Protocols for Experimental Phasing<br>Aaron D. Finke, Ezequiel Panepucci, Clemens Vonrhein, Meitian Wang, Gérard Bricogne, and Vincent Oliéric<br></p><p>12. Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases<br>Huiyan Sun, Sibo Jiang, and Zhen Huang<br></p><p>13. Practical Radiation Damage Induced Phasing<br>Chloe Zubieta and Max H. Nanao<br></p><p>14. Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data<br>Robert T. Batey and Jeffrey S. Kieft<br></p><p>15. Using Molecular Replacement Phasing to Study the Structure and Function of RNA<br>Marco Marcia<br></p><p>16. Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?<br>Alexandre Urzhumtsev, Ludmila Urzhumtseva, and Ulrich Baumann<br></p><p>17. RNA Structure Refinement Using the ERRASER-Phenix Pipeline<br>Fang-Chieh Chou, Nathaniel Echols, Thomas C. Terwilliger, and Rhiju Das<br></p><p>18. Neutron Nucleic Acid Crystallography<br>Toshiyuki Chatake</p><p>Part IV Functional Examples</p><p>19. Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins<br>Sultan Agalarov, Marat Yusupov, and Gulnara Yusupova<br></p><p>20. Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers<br>Jiro Kondo<br></p><p>21. Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor<br>Sergey M. Dibrov and Thomas Hermann<br></p><p>22. Anions in Nucleic Acid Crystallography<br>Luigi D’Ascenzo and Pascal Auffinger</p>
