<p>Part I Review Chapters</p><p>1. Advanced in Plant Gene Silencing Methods<br>Prachi Pandey, Muthappa Senthil-Kumar, and Kirankumar S. Mysore</p><p>2. Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies<br>Christophe Lacomme</p><p>3. Bioinformatics Tools for Achieving Better Gene Silencing in Plants<br>Firoz Ahmed, Xinbin Dai, and Patrick Xuechun Zhao</p><p>4. Profiling of Small RNAs Involved in Plant-Pathogen Interactions<br>Dongdong Niu, Zhaoyun Wang, Shune Wang, Lulu Qiao, and Hongwei Zhao</p><p>5. RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants<br>Abdulrazak B. Ibrahim and Francisco J. L. Aragão</p><p>Part II Methods Chapter</p><p>6. Simplifying Transgene Locus Structure Through Cre-lox Recombination<br>Vibha Srivastava and David W. Ow</p><p>7. Transgene-Induced Gene Silencing in Plants<br>Yun Jin and Hui-Shan Guo</p>8. Gene Silencing by DNA Interference in Fern Gametophytes<br>Masamitsu Wada and Hidenori Tsuboi</p><p>9. Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector<br>Akira Kanazawa and Megumi Kasai</p><p>10. A Method for Validating MicroRNAs in Plants by miR-RACE<br>Jinggui Fang and Xin Sun</p><p>11. MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants<br>Weiwei Chen, Qj Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu, Cheng Qin, Pencheng Zhang, Nongnong Shi, and Yiguo Hong</p><p>12. A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions<br>Senjuti Sinharoy, Catalina I. Pislariu, and Michael K. Udvardi</p><p>13. A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration<br>Vinay Panwar, Brent McCallum, and Guus Bakkeren</p><p>14. An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation<br>Tatsuya Kon and Nubuyuki Yoshikawa</p><p>15. Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector<br>Ravi Kant, Shweta Sharma, and Indranil Dasgupta</p><p>16. Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton<br>John R. Tuttle, Candace H. Haigler, and Dominique Robertson</p><p>17. Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection<br>Ana Marcia E. de A. Manhães, Marcos V. V. de Oliveira, and Libo Shan</p><p>18. Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants<br>Gavin M. George, Michael E. Ruckle, and James R. Lloyd</p><p>19. VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants<br>Mette Grønlund</p><p>20. Construction of a Cotton VIGS Library for Functional Genomic Study<br>Maoying Li, Fangjun Li, and Ping He</p>21. Synthetic Gene Complementation to Determine Off-Target Silencing<br>Dhirendra Kumar</p><p>22. Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants<br>M. E. Chrissie Rey, Johan Harmse, Sarah H. Taylor, Patrick Arbuthnot, and Marc S. Weinberg</p>