Plant Cell Expansion

Methods and Protocols

Specificaties
Gebonden, 231 blz. | Engels
Springer New York | 2015e druk, 2014
ISBN13: 9781493919017
Rubricering
Springer New York 2015e druk, 2014 9781493919017
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists​.

Specificaties

ISBN13:9781493919017
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:231
Uitgever:Springer New York
Druk:2015

Inhoudsopgave

<p>1. Penium margaritaceum as a Model Organism For Cell Wall Analysis Of Expanding Plant Cells</p><p>Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Amanda Andreas, Jesper Harholt, Peter Ulvskov, Bodil Jørgensen, David S. Domozych,  and William G.T. Willats</p><p> </p><p>2. Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum</p><p>N. Worden, V. Esteva Esteve, D. S. Domozych, and G. Drakakaki</p><p> </p><p>3. Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes</p><p>Cecilia Borassi, Juliana Perez Di Giorgio, María R. Scarpin, Jorge Muschietti,<sup> </sup>and  Jose Estevez</p><p> </p><p>4. Imaging of Calcium Dynamics in Pollen Tube Cytoplasm</p><p>María Laura Barberini and Jorge Muschietti</p><p> </p><p>5. Live Imaging of Root Hairs</p><p>Silvia M. Velasquez, Jose R. Dinneny, and <sup> </sup>José M. Estevez<sup></sup></p><p> </p><p>6. Improved ROS Measurement In Root Hair Cells</p><p>Paola Silvina Denita Juarez, Silvina Mangano, adn Jose M. Estevez</p><p> </p><p>7. A Root Hair Assay to Expedite Cell Death Research</p><p>Joanna Kacprzyk and Paul F. McCabe</p><p> </p><p>8. Vacuolar Staining Methods in Plant Cells</p><p>David Scheuring, Maria Schöller, Jürgen Kleine-Vehn, and Christian Löfke</p><p> </p><p>9. Live Cell Imaging Of FM4-64, A Tool For Tracing The Endocytic Pathways In Arabidopsis Root Cells</p><p>Adeline Rigal, Siamsa M. Doyle, and Stéphanie Robert</p><p> </p><p>10. Salt-Stress Regulation Of Root System Growth And Architecture In Arabidopsis Seedlings</p><p>Jose R. Dinneny                    </p><p> </p><p>11. Quantification Of Fluorescent Reporters in Plant Cells</p><p>Michael Pound, Andrew P. French, and Darren M. Wells</p><p> </p><p>12. Live Cell Imaging Of The Cytoskeleton And Cell Wall Enzymes In Plant Cells</p><p>Arun Sampathkumar and Raymond Wightman</p><p> </p><p>13. Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins </p><p>Logan Bashline and Ying Gu </p><p> </p><p> </p><p>14. Activation Tag Screening For Cell Expansion Genes in Arabidopsis thaliana</p><p>Chaowen Xiao and Charles T. Anderson</p><p> </p><p>15. Bifc For Protein-Protein Interactions and Protein Topology: Discussing An Integrative Approach For An Old Technique</p><p>Giovanni Stefano, Luciana Renna, and Federica Brandizzi</p><p> </p><p>16. N-Glycosylation and Plant Cell Growth</p><p>Christiane Veit, Ulrike Vavra, and Richard Strasser</p><p> </p><p>17. Peptide Separation Methodologies for In-depth Proteomics</p><p>Sajad Majeed Zargar, Rie Kurata, Randeep Rakwal, and Yoichiro Fukao</p><p> </p><p>18. Structural And Mechanical Characterization Of Growing Arabidopsis Plant Cell Walls</p><p>Friederike Saxe, Ingo Burgert, and Michaela Eder<sup></sup></p><p> </p>

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