<p>1. Introduction to Membrane Lipids</p><p>Richard M. Epand</p><p> </p><p>2. Introduction: Membrane properties (Good) for Life</p><p>Marek Cebecauer</p><p> </p><p>3. Introduction To Fluorescence Probing Of Biological Membranes</p><p>Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély<sup></sup></p><p> </p><p>4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)</p><p>Charles Cranfield, Sonia Carne, Boris Martinac, <sup> </sup>and Bruce Cornell<sup></sup></p><p> </p><p>5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells</p><p>Deborah A. Brown</p><p> </p><p>6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning</p><p>KR Levental<sup> </sup>and I Levental</p><p> </p><p>7. Asymmetric Giant Lipid Vesicle Fabrication</p><p>Peichi C. Hu and Noah Malmstadt</p><p> </p><p>8. Cholesterol Depletion using Methyl-b-cyclodextrin</p><p>Saleemulla Mahammad and Ingela Parmryd</p><p> </p><p>9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems</p><p>G. Ekin Atilla-Gokcumen and Ulrike S. Eggert<sup> </sup></p><p> </p><p>10. Imaging Membrane Order using Environmentally Sensitive Fluorophores</p><p>G Ashdown and DM Owen<sup></sup></p><p> </p><p>11. 3D Super-resolution Imaging by Localisation Microscopy </p><p>Astrid Magenau and Katharina Gaus</p><p> </p><p>12. Electron Microscopy Methods for Studying Plasma Membranes</p><p>Alison J. Beckett and Ian A. Prior</p><p> </p><p>13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy</p><p>Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas</p><p> </p><p>14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order</p><p>Richard KP Benninger</p><p> </p><p>15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes</p><p>Eduard Hermann, Jonas Ries, and Ana J. García-Sáez</p><p> </p><p>16. X-ray Diffraction of Lipid Model Membranes</p><p>Arwen I.I. Tyler, Robert V. Law, and John M. Seddon </p><p> </p><p>17. Solid State NMR of Lipid Model Membranes</p><p>Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law</p><p> </p><p>18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications</p><p>Michael Carnell, Alex Macmillan, and Renee Whan<sup></sup></p><p> </p><p>19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells</p><p>Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton</p><p> </p><p>20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations</p><p>Semen O. Yesylevskyy and Alexander P. Demchenko<sup></sup></p><p> </p><p>21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers</p><p>Svetlana Baoukina and D. Peter Tieleman </p><p> </p>