Plant Proteostasis

<p>Observing real-time ubiquitination in high throughput with fluorescence polarization.-&nbsp;Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating-Enzymes and E3 Ubiquitin Ligases.-&nbsp;Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis thaliana seedlings.-&nbsp;An in vitro assay to recapitulate hormone-triggered & SCF-mediated protein ubiquitylation.-&nbsp;Analysis of proteasome-associated ubiquitin ligase activity.-&nbsp;Measuring the DUB Activity of Arabidopsis Deubiquitylating enzymes.-&nbsp;SUMO conjugation and SUMO chain formation by plant enzymes.-&nbsp;Kinetic analysis of plant SUMO conjugation machinery.-&nbsp;Expression, purification, and enzymatic analysis of plant SUMO proteases.-&nbsp;Monitoring autophagic flux in the model single-celled microalga Chlamydomonas reinhardtii.-&nbsp;Detection of autophagy in plants by fluorescence microscopy.-&nbsp;Characterization of ATG8-family interactors by Isothermal Titration Calorimetry.-&nbsp;Protocols for studying protein stability in a Arabidopsis protoplast transient expression system.-&nbsp;Relative Protein Lifetime Measurement in Plants using Tandem Fluorescent Protein Timers.-&nbsp;Detection and Quantification of Protein Aggregates In Plants.-&nbsp;Using intrinsic fluorescence to measure&nbsp;protein&nbsp;stability upon thermal and chemical denaturation.-&nbsp;Purification and detection of ubiquitinated plant proteins using Tandem Ubiquitin Binding Entities.-&nbsp;Titanium oxide-based phosphopeptide enrichment from Arabidopsis seedlings.-&nbsp;Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea.-&nbsp;Chromatin Enrichment for Proteomics in Plants (ChEP-P).-&nbsp;Proximity-dependent in vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha.-&nbsp;Tandem mass tag-based phospho-proteomics in plants.-&nbsp;Analysis of peptide hormone maturation and processing specificity using isotope-labeledpeptides.-&nbsp;Improved identification of protease cleavage sites by in-gel reductive dimethylation.-&nbsp;A Pipeline to Monitor Proteasome Homeostasis in Plants.-&nbsp;Bioinformatics tools for exploring the SUMO Gene Network, an update.-&nbsp;Coexpression network constriction and visualization from transcriptomes underlying ER stress responses.-&nbsp;Approaches for the identification of intrinsically disordered protein domains.</p>

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