Tumor Angiogenesis Assays

Discovery and development of tumor angiogenesis assays.-&nbsp;The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino.-&nbsp;Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry.-&nbsp;Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels.-&nbsp;Visualizing the interactions that haping the imaging of microenvironment in cancer diseases.-&nbsp;Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach.-&nbsp;Monitoring angiotropic extravascular migratory metastasis in vitro.-&nbsp;Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option.-&nbsp;Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment.-&nbsp;IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM).-&nbsp;Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model.-&nbsp;The chick CAM as an in vivo system to study stem cell activity.-&nbsp;Detection of possible symmetries in vascular networks by computer assisted image analysis.-&nbsp;Alternative in vivo models to study teratoma.-&nbsp;How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation.-&nbsp;3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models.<p><br></p>