Complete Visualization of T Follicular Helper Cells in Germinal Centers.- In vivo imaging of Tfh cells.- Circulating T Follicular Helper Subsets in Human Blood.- Identification of Human Circulating and Tissue T Follicular Regulatory (Tfr) Cells by Flow Cytometry.- Assessment of circulating Tfh and Tfr by mass cytometry.- Identification of T peripheral helper (Tph) cells.- Identification of non-classical follicular T cells.- Identification of follicular T cells in the gut.- Intravital imaging of Tfh dynamics.- A method to analyze models of the dynamics of GCs.- Identification of Antigen Presenting Cell subsets supporting human Tfh differentiation Lamine Alaoui, Melanie Durand, and Elodie Segura.- The use of peptide-MHCII tetramers to identify antigen-specific T follicular helper and T follicular regulatory cells.- Assessment of the TCR repertoire of human circulating T follicular helper cells.- Assessing in vivo T cell-dependent antigen-specific antibody responses.- Tfh-mediated and Tfr-suppressed antigen driven IgG and IgE assays.- Assessment of the impact of cytokines on TFH, TREG, and TFR cell populations after influenza infection.- Evaluation of human circulating T follicular helper cells in influenza- and SARS-CoV-2-specific B cell immunity.- Detection of T follicular helper cells and T follicular regulatory cells in experimental Sjögren’s syndrome.- Identification of Human T Follicular Cells in Ectopic Lymphoid Structures.- Understanding T Follicular helper cells in asthma through murine models of allergic airway disease.- Therapeutic modulation of T follicular helper cells by low-dose IL-2 treatment.- 3D tissue explant and single-cell culture systems for ex vivo drug testing on Tfh cells derived from human adenoid tonsils.<p><br></p>
