,

Enhancers and Promoters

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer US | e druk, 2021
ISBN13: 9781071615966
Rubricering
Springer US e druk, 2021 9781071615966
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting.Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments.
Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Specificaties

ISBN13:9781071615966
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

Inhoudsopgave

A New Toolbox to Analyze Enhancer-Promoter Functions.-&nbsp;Global Run-on Sequencing (GRO-Seq).-&nbsp;Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq).-&nbsp;Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters.-&nbsp;Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq.-&nbsp;Measuring Chromatin Accessibility: ATAC-Seq.-&nbsp;High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.-&nbsp;Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.-&nbsp;Low Input Targeted Chromatin Capture (low-T2C).-&nbsp;Proximity Ligation-assisted ChIP-Seq (PLAC-Seq).-&nbsp;Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies.-&nbsp;Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions.-&nbsp;Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C).-&nbsp;Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.-&nbsp;Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin.-&nbsp;Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq).-&nbsp;Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.-&nbsp;Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors.-&nbsp;Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing.-&nbsp;Low Input Whole-Genome Bisulfite Sequencing.<p><br></p>

Rubrieken

    Personen

      Trefwoorden

        Enhancers and Promoters