Staphylococcus aureus

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer US | e druk, 2021
ISBN13: 9781071615492
Rubricering
Springer US e druk, 2021 9781071615492
Onderdeel van serie Methods in Molecular Biology
€ 180,99
Levertijd ongeveer 8 werkdagen

Samenvatting

This volume details the phenotypic characterization of Staphylococcus aureus, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Staphylococcus aureus: Methods and Protocols is a valuable resource for anyone interested in this fascinating and developing field.

Specificaties

ISBN13:9781071615492
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

Inhoudsopgave

<div>Measurement of Staphylococcus aureus Pigmentation by Methanol Extraction.-&nbsp;Analysis of Murein Hydrolases and Proteases through Zymography.-&nbsp;Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants.-&nbsp;Quantitative Hemolysis Assays.-&nbsp;In Vitro Assay for Quantifying Clumping of Staphylococcus aureus.-&nbsp;Measuring Staphylococcal Promoter Activities using a Codon-Optimized β-galactosidase Reporter.- Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays.-&nbsp;Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity.-&nbsp;Quantification of Staphylococcus aureus Biofilm Formation by Crystal&nbsp;Violet and Confocal Microscopy.-&nbsp;Growth of Staphylococcus aureus using a Rotary Cell Culture System.-&nbsp;Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus.-&nbsp;Quantifying Staphylococcus aureus Membrane Potentialusing Flow Cytometry.-&nbsp;Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples.-&nbsp;A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins.-&nbsp;An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood.-&nbsp;Bone Marrow-Derived Macrophage Infection Assay.-&nbsp;Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins.-&nbsp;Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm.</div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><br><div><br></div>
€ 180,99
Levertijd ongeveer 8 werkdagen

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        Staphylococcus aureus