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Patch Clamp Electrophysiology

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer US | e druk, 2020
ISBN13: 9781071608173
Rubricering
Springer US e druk, 2020 9781071608173
Onderdeel van serie Methods in Molecular Biology
€ 240,99
Levertijd ongeveer 8 werkdagen

Samenvatting

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Patch Clamp Electrophysiology:  Methods and Protocols aims to be a reference guide for current and future ion channel physiologists. 

Specificaties

ISBN13:9781071608173
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

Inhoudsopgave

<p>An Introduction to Patch Clamp Recording.-&nbsp;Patch Clamp Technology in the 21st Century.-&nbsp;Heterologous Expression of Ion Channels in Mammalian Cell Lines.-&nbsp;Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel.- Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology.-&nbsp;Multi-electrode Arrays.-&nbsp;Utilising Automated Electrophysiological Platforms in Epilepsy Research.-&nbsp;Dynamic Clamp on a Windows PC.-&nbsp;Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing.-&nbsp;Bioluminescence Methodology for Ion Channel Studies.-&nbsp;Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons.-&nbsp;Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method.-&nbsp;In Vivo Patch Clamp Studies.-&nbsp;&nbsp;In Vivo Optogenetics with Stimulus Calibration.-&nbsp;Single or Double Patch ClampRecordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics.-&nbsp;Optogenetics and Optical Tools in Automated Patch Clamping.</p>

<br> <p>&nbsp;</p>
€ 240,99
Levertijd ongeveer 8 werkdagen

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        Patch Clamp Electrophysiology