What′s Wrong With My Mouse? – Behavioral Phenotyping of Transgenic and Knockout Mice 2e
Behavioral Phenotyping of Transgenic and Knockout Mice
Samenvatting
Dr. Jacqueline N. Crawley, author of the First and Second Editions of
What s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e–mails from molecular geneticists who ask:
how do I run behavioral assays to find out what s wrong with my mouse?
Turn to What s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters:
"Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis.
"Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
The final chapter, "The Next Generation," examines new and emerging technologies.
Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step–by–step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.
Specificaties
Inhoudsopgave
<p>Preface to the First Edition xi</p>
<p>Acknowledgments xiii</p>
<p>Chapter 1 Designer Mice 1</p>
<p>Scope and sourcebooks</p>
<p>Chapter 2 Of Unicorns and Chimeras 10</p>
<p>How to generate a line of transgenic or knockout mouse for behavioral phenotyping</p>
<p>Chapter 3 General Health 42</p>
<p>Give your mouse a physical</p>
<p>Chapter 4 Motor Functions 62</p>
<p>Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures</p>
<p>Chapter 5 Sensory Abilities 86</p>
<p>Olfaction, vision, hearing, taste, touch, nociception</p>
<p>Chapter 6 Learning and Memory 110</p>
<p>Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention</p>
<p>Chapter 7 Feeding and Drinking 164</p>
<p>Daily consumption, restricted access, choice tests, microstructural analysis</p>
<p>Chapter 8 Reproductive Behaviors 186</p>
<p>Sexual and parental behaviors</p>
<p>Chapter 9 Social Behaviors 206</p>
<p>Social interaction, nesting, grooming, juvenile play, aggression</p>
<p>Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226</p>
<p>Mouse models of fear, anxiety, depression, schizophrenia</p>
<p>Chapter 11 Reward 266</p>
<p>Self–administration of addictive drugs, conditioned place preference</p>
<p>Chapter 12 Neurodevelopment and Neurodegeneration 290</p>
<p>Assaying behaviors in infant, juvenile, and aged mice</p>
<p>Chapter 13 Putting It All Together 322</p>
<p>Choice of tests, order of testing, number of mice, equipment, housing and testing environment</p>
<p>Chapter 14 The Next Generation 344</p>
<p>Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues</p>
<p>References 383</p>
<p>Index 503</p>
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