Immunophenotyping
Samenvatting
Immunophenotyping Edited by Carleton C. Stewart and Janet K. A. Nicholson In the last twenty years there has been an explosion of immunophenotyping applications using flow cytometry in the clinical laboratory. Immunophenotyping offers essential information for clinicians and laboratorians about the uses of flow cytometers, identifying abnormalities in a variety of disorders, tools of immunophenotyping, assessing platelets in disease states, and much more. This second volume in the new series, Cytometric Cellular Analysis, comprises all cytometric methods currently used to study cellular function through reviews of the principles, theoretical background, and applications of these methods with particular reference to their use in clinical laboratories. Cellular analytical technologies have revolutionized our ability to identify, isolate, and functionally characterize single cells. This volume addresses one of the most important aspects of flow cytometry as it applies to the clinical setting. In addition, this comprehensive book:
∗ Discusses methods used for quality controlling immunophenotyping trials
∗ Reviews various approaches to the use of flow cytometers to quantify fluorescence
∗ Explains how cell surface receptors differentiate between normal and abnormal tissues for diseases including lymphoma, leukemia, and AIDS
∗ Describes a new, sensitive flow cytometric immunophenotyping assay for cross–matching in the case of transplants
Imraunophenotyping conveys to researchers, lab supervisors, and scientists working in the areas of flow cytometry, cytology, pathology, hematotogy, immunology, and immunopathology why this outstanding technology is so vital to biomedical research.
Specificaties
Inhoudsopgave
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<br /> Contributors
<br />
<br /> 1 Kinetics of Antibody Binding to Cells
<br />
<br /> Carleton C. Stewart and George L. Mayers
<br />
<br /> 2 Flow Cytometry and Quality Control: An Uneasy Alliance
<br />
<br /> Marc Langweiler and Alice L. Givan
<br />
<br /> 3 Flow Cytometric Analyses of Cell Phenotypes
<br />
<br /> Doug Redelman
<br />
<br /> 4 Quantitative Immunophenotyping
<br />
<br /> Abraham Schwartz and Emma Fernández–Repollet
<br />
<br /> 5 Clinical Applications of Quantitative Immunophenotyping
<br />
<br /> Philippe Poncelet, Isabelle Besson–Faure and Thierry Lavabre–Bertrand
<br />
<br /> 6 Normal Antigen Expression in Hematopoiesis: Basis for Interpreting Leukemia Phenotypes
<br />
<br /> Michael R. Loken and Denise A. Wells
<br />
<br /> 7 Clinical Applications of Flow Cytometric Immunophenotyping in Acute Lymphoblastic Leukemia
<br />
<br /> Joseph A. DiGiuseppe and Michael J. Borowitz
<br />
<br /> 8 Immunotyping of Chronic Lymphoid Leukemias and Related Non–Hodgkin′s Leukemias
<br />
<br /> John L. Carey
<br />
<br /> 9 Use of Multiparameter Flow Cytometry and Immunophenotyping for the Diagnosis and Classification of Acute Myeloid Leukemia
<br />
<br /> Gregory T. Stelzer and Lindsley Goodpasture
<br />
<br /> 10 Residual Disease Detection of Leukemia
<br />
<br /> Alberto Orfao, Juana Ciudad, Julia Almeida, and Jesus F. San Miguel
<br />
<br /> 11 Immunophenotyping in HIV Infection
<br />
<br /> Janet K.A. Nicholson and Francis F. Mandy
<br />
<br /> 12 Enumeration of CD34–Positive Hematopoietic Progenitor Cells
<br />
<br /> Adrian P. Gee and Lawrence S. Lamb
<br />
<br /> 13 Transplantation Immunophenotyping
<br />
<br /> Robert A. Bray and Howard M. Gebel
<br />
<br /> 14 Flow Cytometric Analysis of Platelets and Platelet Function
<br />
<br /> T. Vincent Shankey, Walter P. Jeske, and Jeanine M. Walenga
<br />
<br /> 15 Flow Cytometric Analysis of Intracellular Protein Epitopes
<br />
<br /> James W. Jacobberger
<br />
<br /> 16 Combined Immunophenotyping and Molecular Phenotyping
<br />
<br /> Charles L. Goolsby, Elizabeth Thompson, and Victoria L. Mosiman
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<br /> Index