TRNA-derived RNAs

1. Purification of endogenous tDRs by hybridization-based pulldown<br>Yasutoshi Akiyama<br>2. Northern blotting for human pre-tRNA and tRNA-derived RNAs<br>Yasutoshi Akiyama<br>3. In vitro translation systems to study tDRs<br>Pavel Ivanov<br>4. tRNA derived RNAs that form tetramolecular assemblies<br>Prakash Kharel<br>5. Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria<br>Norbert Polacek<br>6. Ribosome-associated tDRs in yeast<br>Norbert Polacek<br>7. tDR bioinformatics<br>Todd Lowe<br>8. cP-RNA-seq for the specific sequencing of tRNA halves and other cP-containing RNAs<br>Yohei Kirino<br>9. TaqMan RT-qPCR for specific quantification of tRNA halves and other small RNAs<br>Yohei Kirino<br>10. Detection and isolation extracellular tDRs<br>Alfonso Cayota<br>11. Preparation of nicked tRNAs<br>Juan Pablo Tosar and Alfonso Cayota<br>12. In vitro functional analysis of plant tDRs<br>Laurence Drouard<br>13. TGIRT-seq to profile tRNA-derived RNAs and associated RNA modifications<br>Anindya Dutta, Zhangli Su, Abigail Johnston and Monima Anam<br>14. A general framework to over-express tRNA-derived RNAs from their parental tRNAs in mammalian cells<br>Anindya Dutta, Zhangli Su, Kumarjeet Banerjee and Fatemeh Esmaeili<br>15. Functional characterization of tRNA-derived small RNAs in stem cells<br>Cristian Bellodi<br>16. Detection of mitochondrial tDRs in killifish<br>Claire Riggs, Gazal Kalyan and Jason Podrabsky<br>17. Measurement and comparison of full-length tRNA and tRF abundance and modifications<br>Tao Pan and Luke Frietze<br>18. Using a Fluorescence based tRNA to image tDR biogenesis and localization.<br>Saumya Das and Guoping Li<br>19. Manipulation of tDR expression using gain of function and loss of function approaches<br>Saumya Das and Guoping Li<br>20. TBD<br>Mark A. Kay and Hak Kyun Kim<br>21. Nicked tRNAs in bacteria<br>Sandra Wolin<br>22. Determining Small RNA-Interacting Proteomes Using Endogenously Modified tRNA-Derived RNAs<br>Matthias Ronald Schaefer, Vera Oberbauser and Aleksej Drino<br>23. Activation of angiogenin by ribosome to produce tRNA-derived RNAs<br>Andrei A. Korostelev and Anna B. Loveland