1. Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation<br>Douglas McNeel<br>2. Cancer cyro-immunotherapy: Models and methods<br>George Prendergast<br>3. Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology<br>Ileana Mauldin and Leon Zheng<br>4. In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes<br>Mario Tarantini, Anna Lisa GIULIANI, Valentina Vultaggio-Poma, Simonetta Falzoni, Elena Adinolfi and Francesco Di Virgilio<br>5. Analysis of the tumor microenvironment using imaging mass cytometry data<br>Zlatko Trajanoski and Zuzana Loncova<br>6. Saphenous vein blood collection for different immune analyses of living mice<br>Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla and Kassandra Ofelia Rodriguez-Aguillon<br>7. Use of mouse KO models to validate the specificity of monoclonal antibodies<br>George Prendergast, Lisa D. Laury-Kleintop, Summer Sedano and Kaylend Manley<br>8. Imaging mass cytometry to analyze the immune TME<br>Louis M. Weiner<br>9. Autoimmune arthritis: Transgenic mouse models and methods<br>Laura Mandik-Nayak, Lauren Merlo and Weidan Peng<br>10. Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments<br>Maha Ayyoub, Clara Maria SCARLATA, Virginie Feliu and Christel Devaud<br>11. Generation and functional evaluation of bispecific antibodies<br>Luis Alvarez-Vallina, Antonio Tapia Galisteo, Anaïs Jiménez-Reinoso, Rodrigo Lázaro Gorines and Belén Blanco<br>12. Isolation and phenotype analysis of intraepithelial lymphocytes from the mouse colon<br>Laura Bracci, Alessia Fiore, Michela Tozzi, Paola Rinzo, Daniele Macchia, Massimo Spada and Alessia Fabbri<br>13. Analysis of single cell TCR repertoires and gene expression from multi-modal scRNA-seq data<br>Dietmar Rieder, Christina Plattner and Gregor Sturm<br>14. Analysis of the tumor immune microenvironment of brain tumors by flow-cytometry<br>Claire I. Vanpouille-Box<br>15. Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time-lapse microscopy.<br>Fabrizio Mattei, Giovanna Schiavoni, Caterina Antonucci, Adriana Rosa Gambardella and Valentina Tirelli<br>16. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells<br>Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
