Integrated Methods in Protein Biochemistry: Part A

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2022
ISBN13: 9780323992664
Rubricering
Elsevier Science e druk, 2022 9780323992664
Onderdeel van serie Methods in Enzymology
€ 178,50
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Samenvatting

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.

Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

Specificaties

ISBN13:9780323992664
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

1. DNA and protein engineering to create protein bioswitches with new functions<br>Stewart Loh<br>2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases<br>Susan Michaelis<br>3. An experimental protocol to study lipid transfer proteins<br>Haijia Yu<br>4. Synthesis of small heat shock proteins<br>Matthew Pratt<br>5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins<br>Guang-Yu Yang<br>6. An experimental protocol for glycoconjugate analysis<br>Yifa Zhou<br>7. Methods for proximity-based biotinylation combined with Mass Spectrometry<br>Ralph Kehlenbach<br>8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking<br>Arun K. Shukla<br>9. Expression, purification, functional analysis and crystallization of Rag GTPase<br>Kuang Shen<br>10. Purification of bacterial transcription elongation complexes by photoreversible immobilization<br>Eric Strobel<br>11. Inhibition of c-Myc-MAX heterodimerization<br>Neha Garg<br>12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases<br>Markus C. Wahl<br>13. Phosphorylation and subcellular localization of human phospholipase A1<br>Atsushi Yamashita<br>14. Integrated methods in studying light-sensitive proteins<br>Lars-Oliver Essen<br>15. Structure and biochemistry of thermophilic protein<br>Ankan Banerjee<br>16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure<br>Gert Bange<br>17. Nanopore force spectroscopy to probe structural dynamics of proteins<br>Sonja Schmid<br>18. Structural analysis of knotted proteins<br>Shang-Te "Danny" Hsu<br>19. Mutation-induced structural changes in the SARC-CoV2 spike protein<br>Shang-Te "Danny" Hsu<br>20. Preparing metabolite capped RNA substrates using RNA polymerases<br>Jeremy G. Bird
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        Integrated Methods in Protein Biochemistry: Part A