Integrated Methods in Protein Biochemistry: Part B

1. Precise modification of native proteins, purification, and analysis of bioconjugates <br>2. NanoBiT-based methods to monitor the activation and modulation of RTKs <br>3. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling <br>4. Structure and function of bacterial secretion system <br>5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase <br>6. Tools and protocols for probing protein sumoylation <br>7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase <br>8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms <br>9. Covalent protein painting: MS-based protein footprinting <br>10. Characterization of GPCR signaling complexes using negative-staining electron microscopy <br>11. Probing protein misfolding and dissociation with free electron laser <br>12. Optimized protocol for the characterization of Cas12a activities <br>13. Proximity proteomics for the identification and characterization of extracellular vesicles <br>14. Structural and Functional characterization of lytic polysaccharide monooxygenases <br>15. Characterization of RRE domain in RiPP biosynthesis <br>16. Structural and mechanistic insights into ferrous iron import and export systems <br>17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging <br>18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry <br>19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins <br>20. Integrated studies on ageing-related proteins extending life span in yeast <br>21. An optimized pipeline for global selenoproteome analysis <br>22. High-throughput screening and evolution of carbohydrate-active enzymes <br>23. Regulation of enzymes with light using unnatural amino acids <br>24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies <br>25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics <br>26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors <br>27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules <br>28. Reconstitution of the MLL1 complex and probing methyltransferase activity <br>29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy <br>30. Structural and functional analysis of mono-copper enzymes from fungal species