Coenzyme B12 Enzymes Part B

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2022
ISBN13: 9780323955577
Rubricering
Elsevier Science e druk, 2022 9780323955577
Onderdeel van serie Methods in Enzymology
€ 178,50
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Samenvatting

Coenzyme B12 Enzymes, Part B, Volume 169 in the Methods in Enzymology series, highlights new advances in the field,  with this new volume presenting interesting chapters on Structural characterization of cobalamin-dependent radical SAM methylases, Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis, Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis, Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis, Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB, and more.

Other chapters discuss Methods for studying the mechanisms of B12 enzymes, Computational investigations of B12 dependent enzymatic reactions, Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes, Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase, and more.

Specificaties

ISBN13:9780323955577
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>B<SUB>12</SUB>-dependent radical SAM enzymes<br>1. Structural characterization of cobalamin-dependent radical SAM methylases<br>Squire Booker Penn State University<br>2. Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis<br>Craig Townsend Johns Hopkins University<br>3. Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis<br>Tadashi Eguchi Tokyo Institute of Technology<br>4. Studies of OxsB and GenK, two B<SUB>12</SUB>-dependent radical SAM enzymes involved in natural product biosynthesis<br>Hung-Wen Liu University of Texas, Austin<br>5. Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB<br>Jennifer Bridwell-Rabb University of Michigan</p> <p>Methods for studying the mechanisms of B<SUB>12</SUB> enzymes<br>6. Computational investigations of B<SUB>12</SUB>-dependent enzymatic reactions<br>Pawel Kozlowski University of Louisville, Kentucky<br>7. Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes<br>Neil Marsh University of Michigan<br>8. Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase<br>Kirsten Wolthers University of British Columbia<br>9. Structural characterization of radical intermediates in reaction of lysine 5,6-aminomutase by EPR, ENDOR, and ESEEM<br>Shyue-Chu Ke National Dong Hwa University, Taiwan<br>10. Solvent-protein-reaction dynamical coupling in B<SUB>12</SUB> enzyme catalysis<br>Kurt Warncke Emory University, Atlanta<br>11. Investigating radical pair reaction dynamics of B<SUB>12</SUB> coenzymes using magnetic field effects<br>Alex Jones National Physical Laboratory, U.K.<br>12. Investigating radical pair reaction dynamics of B<SUB>12</SUB> coenzymes using time-resolved electron paramagnetic resonance spectroscopy<br>Alex Jones National Physical Laboratory, U.K.<br>13. Time-resolved spectroscopy: Advances in understanding the electronic structure and dynamics of cobalamins<br>Roseanne Sension and James enner-Hahn University of Michigan<br>14. MCD and Raman Spectroscopy of cobalamin-dependent enzymes<br>Thomas Brunold University of Wisconsin, Madison</p>
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        Coenzyme B12 Enzymes Part B