<p>1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New Therapies<br>Xiaoyan Jiang<br>2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR<br>Guillermo Mazzolini<br>3. Reverse Phase Protein Array in cancer stem cells<br>Michele Signore<br>4. Isolation of cancer stem cells from squamous cell carcinoma<br>Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC),<br>5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells<br>Niccolò Bartalucci<br>6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity<br>Paola Marcato<br>7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs)<br>Angela Gritti<br>8. Immunohistochemistry-based analysis of EMT markers in mammary tumors<br>Diwakar Pattabiraman<br>9. Immunofluorescence staining of colorectal cancer patient-derived organoids<br>Joseph L. Regan<br>10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry<br>Michael A. Rieger<br>11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells<br>Bin Wang Sr., Yingying Lai and Xi Zheng<br>12. Methods to isolate adipose tissue-derived stem cells<br>Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco<br>13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells<br>Antonella Sistigu</p>